中成药2025，Vol.47Issue(7)：2190-2197,8.DOI:10.3969/j.issn.1001-1528.2025.07.009
薯蓣丸联合依维莫司对TGF-β1诱导三阴性乳腺癌细胞上皮间质转化的抑制作用
Inhibitory effects of Shuyu Pills combined with everolimus on TGF-β1-induced epithelial-mesenchymal transition in triple-negative breast cancer cells
摘要
Abstract
AIM To investigate the effects of Shuyu Pills combined with evolimus on the epithelial mesenchymal transformation of triple negative breast cancer cells 4T1 and MDA-MB-231 induced by TGF-β1.METHODS The 4T1 and MDA-MB-231 cells were divided into the blank group and the induction group to induce the epithelial mesenchymal transformation with TGF-β1 cytokine treatment,followed by the assignment into the model group,the Shuyu Pills group,the everolimus group and the Shuyu Pills combined with everolimus group.CCK8 method,plate cloning method,cell scratch test and Transewll test were used to detect the proliferation,cloning formation,migration and invasion ability of the cells whose expressions of E-cadherin,N-cadherin,Vimentin,MMP9,MMP2 and pathway proteins PTEN,PI3K,Akt and mTOR were detected by Western blot.RESULTS Compared with the blank group,the induction group displayed a cell morphological change from epithelioid to stromal,decreased expression of E-cadherin protein(P＜0.01);and increased protein expressions of N-cadherin and Vimentin(P＜0.05).Compared with the model group,each group intervened with the medicine displayed decreased proliferation,clone formation,migration and invasion ability of both kinds of cells(P＜0.01);increased protein expressions of PTEN and E-cadherin(P＜0.05,P＜0.01);and decreased protein expressions of PI3K,Akt,mTOR,N-cadherin,Vimentin,MMP9 and MMP2(P＜0.05,P＜0.01);and more significantly in the Shuyu Pills combined with evolimus group(P＜0.05,P＜0.01).CONCLUSION With a more ideal effect than the single uses in inhibiting the TGF-β1-induced epithelial mesenchymal transformation of triple-negative breast cancer cells,the combination use of Shuyu Pills and everolimus may work through the regulation of PI3K/Akt/mTOR signaling pathway.关键词
薯蓣丸/依维莫司/三阴性乳腺癌/TGF-β1/上皮间质转化/PI3K/Akt/mTOR信号通路Key words
Shuyu Pills/everolimus/triple negative breast cancer/TGF-β1/epithelial mesenchymal transformation/PI3K/Akt/mTOR signaling pathway分类
医药卫生引用本文复制引用
骆小珊,谢甦,冯豆豆,蒙雁云,赵晶哲,谢青,凌湘力..薯蓣丸联合依维莫司对TGF-β1诱导三阴性乳腺癌细胞上皮间质转化的抑制作用[J].中成药,2025,47(7):2190-2197,8.基金项目
国家自然科学基金(81960834) （81960834）
凌湘力全国名中医传承工作室建设项目(国中医药办人教函[2022]245号) （国中医药办人教函[2022]245号）
贵州省科技计划项目(黔科合基础-ZK[2022]一般442,黔科合基础-ZK[2024]一般204) （黔科合基础-ZK[2022]一般442,黔科合基础-ZK[2024]一般204）
贵州省中医药管理局项目(QZYY-2023-001) （QZYY-2023-001）
