中成药2025，Vol.47Issue(7)：2198-2205,8.DOI:10.3969/j.issn.1001-1528.2025.07.010
白头翁合理中汤加减通过调控AMPK/mTOR/p70S6K信号通路介导的自噬对小鼠溃疡性结肠炎的改善作用
Effects of Modified Baitouweng Decoction and Lizhong Decoction on the improvement of mouse ulcerative colitis through autophagy regulation via AMPK/mTOR/p70S6K signaling pathway
摘要
Abstract
AIM To investigate the effects of Modified Baitouweng Decoction and Lizhong Decoction on mouse ulcerative colitis(UC).METHODS The mouse model of UC was established by 3％dextran sulfate sodium(DSS)induction.The C57BL/6 mice were randomly divided into the blank group,the model group,the low,medium and high dose Modified Baitouweng Decoction and Lizhong Decoction groups(3,6,12 g/kg),and sulfasalazine group(300 mg/kg),for 7 days gavage of the appropriate drugs,with 10 mice in each group.The mice had their disease activity index(DAI)and colonic mucosal damage index(CMDI)calculated;their colonic length and unit colonic weight measured;their histopathologic changes of colon observed by HE;their colonic ROS,MDA levels and GSH-Px,SOD activities detected by superoxide anion fluorescent probes and kits;their colonic levels of TNF-α,IL-6 and IL-1β detected by ELISA;their colonic LC3 expression detected by immunofluorescence method;and their colonic AMPK,mTOR and p70S6K protein expressions detected by Western blot method.RESULTS Compared with the blank group,the model group displayed significantly higher DAI score,CMDI score,unit colon weight,pathology score,ROS and MDA content,TNF-α,IL-6,and IL-1β levels,and mTOR and p70S6K protein expression(P＜0.01);and significantly lower colon length,GSH-Px and SOD activity,LC3 level,and phosphorylated AMPK protein expression(P＜0.01).Compared with the model group,the groups intervened with Modified Baitouweng Decoction and Lizhong Decoction or sulfasalazine shared decreased DAI score,CMDI score,unit colon mass,pathology score,ROS,MDA,TNF-α,IL-6,IL-1β levels,mTOR,p70S6K protein expressions(P＜0.01);and significantly improved symptomsin terms of the elevated colonic length,GSH-Px,SOD activities,LC3 level,AMPK protein expression(P＜0.01).CONCLUSION Modified Baitouweng Decoction and Lizhong Decoction may attenuate inflammatory response and oxidative damage in UC mouse models via AMPK/mTOR/p70S6K signaling pathway.关键词
白头翁合理中汤/溃疡性结肠炎/AMPK/mTOR/p70S6K信号通路/自噬Key words
Baitouweng Decoction and Lizhong Decoction/ulcerative colitis/AMPK/mTOR/p70S6K signaling pathway/autophagy分类
医药卫生引用本文复制引用
张亚粉,左振魁,菅洪磊,王琳,刘天瑞,任兰鑫..白头翁合理中汤加减通过调控AMPK/mTOR/p70S6K信号通路介导的自噬对小鼠溃疡性结肠炎的改善作用[J].中成药,2025,47(7):2198-2205,8.基金项目
河南省中医药拔尖人才培养项目(2022ZYBJ11) （2022ZYBJ11）
河南省中医药科学研究专项课题(2019ZY2022) （2019ZY2022）
河南省研究生教育改革与质量工程项目(YJS2021AL059) （YJS2021AL059）
