植物保护2025，Vol.51Issue(4)：39-52,14.DOI:10.16688/j.zwbh.2024430
斜纹夜蛾SlbHLH互作蛋白的筛选与验证
Screening and validation of SlbHLH-interacting proteins in Spodoptera litura
摘要
Abstract
To explore the role of the bHLH transcription factor in the response of Spodoptera litura to microbial pesticide stress,yeast two-hybrid assay was carried out to screen the cDNA library constructed from microbial pesticide-treated S.litura using SlbHLH as the bait.Through sequencing and BLAST analysis,a total of 38 candidate interacting proteins were initially identified.Point-to-point yeast two-hybrid assays confirmed the interactions between SlbHLH and SlCDA,SlCHIT,SlFCP,SlHEMO,SlLCP,and SlTRYP.RT-qPCR analysis following treatment with Metarhizium anisopliae,Empedobacter brevis and Bacillus thuringiensis revealed that the expression patterns of SlbHLH were consistent with those of SlCHIT,SlFCP,SlHEMO,and SlLCP,but opposite to that of SlTRYP under specific microbial pesticide stresses and treatment conditions.These results suggest that SlbHLH and its interacting proteins may collaboratively regulate the response of S.litura to microbial pesticide stress.This study provides a foundation for further elucidating the biological functions of the bHLH transcription factor and its interacting proteins in S.litura.关键词
斜纹夜蛾/bHLH转录因子/酵母双杂交/互作蛋白/基因表达Key words
Spodoptera litura/bHLH transcription factor/yeast two-hybrid/interacting protein/gene expression分类
农业科技引用本文复制引用
吴霜,罗云米,曾志红,王瑢笙,余鹰,陈磊..斜纹夜蛾SlbHLH互作蛋白的筛选与验证[J].植物保护,2025,51(4):39-52,14.基金项目
重庆市农业科学院市级财政科研项目(cqaas2023sjczzd011) （cqaas2023sjczzd011）
重庆市自然科学基金(CSTB2023NSCQ-MSX0608) （CSTB2023NSCQ-MSX0608）
