植物保护2025，Vol.51Issue(4)：53-62,130,11.DOI:10.16688/j.zwbh.2024395
二酰甘油酰基转移酶1(DGAT1)基因促进滞育态七星瓢虫的脂质积累
Diacylglycerol-O-acyltransferase 1 gene promotes lipid accumulation in diapausing Coccinella septempunctata
摘要
Abstract
Coccinella septempunctata is widely used predatory natural enemy insect in biological pest control.It survive the winter as a diapause adult,during which females accumulate substantial abdominal lipids,significantly prolonging survival.Lipid accumulation is a feature of insect diapause,supplying energy and nutrients for basic metabolism during the diapause period and post-diapause recovery,thereby enhancing survival under unfavorable conditions.Previous studies revealed that triacylglycerol(TAG)is the main lipid form in diapause females of C.septempunctata,with significantly increased TAG levels.Diacylglycerol-O-acyltransferase(DGAT),the terminal and rate-limiting enzyme in TAG synthesis,remains poorly studied in insects.In this study,we identified a gene encoding DGAT1(CsDGAT1)based on the genome of C.septempunctata.The full-length ORF of CsDGAT1 was cloned and analyzed via bioinformatics.RT-qPCR was used to examine its expression profile in diapause-induced and non-diapause females,and RNAi was employed to knock down CsDGAT1 expression and assess the effect on TAG content.Phylogenetic analysis showed that CsDGAT1 is closely related to DGAT1 from Harmonia axyridis and contains eight conserved histidine residues.Under non-diapause conditions,CsDGAT1 was highly expressed in the fat body and ovary;under diapause-induced conditions,it was primarily expressed in the fat body and midgut.CsDGAT1 expression was significantly upregulated in fat body and midgut of diapause-induced females compared to non-diapause females.RNAi-mediated silencing of CsDGAT1 significantly reduced TAG levels and fat accumulation.These results demonstrate that the CsDGAT1 plays an important role in lipid accumulation in diapausing females,providing a molecular basis for understanding diapause-related lipid metabolism and a theoretical foundation for developing diapause-induction technologies for natural enemy insects in biological control.关键词
七星瓢虫/二酰甘油酰基转移酶1/脂质积累/滞育/表达特征/RNA干扰Key words
Coccinella septempunctata/diacylglycerol-O-acyltransferase 1/lipid accumulation/diapause/gene expression/RNA interference分类
农业科技引用本文复制引用
陈俊杰,韩舜达,董文静,郭鹏辉,刘晓晓,刘照涵,张礼生..二酰甘油酰基转移酶1(DGAT1)基因促进滞育态七星瓢虫的脂质积累[J].植物保护,2025,51(4):53-62,130,11.基金项目
国家重点研发计划(2023YFD1400600) （2023YFD1400600）
中国农业科学院科技创新计划(CAAS-ZDRW202203) （CAAS-ZDRW202203）
中国博士后科学基金(2024M763614) （2024M763614）
