植物保护2025，Vol.51Issue(4)：63-71,9.DOI:10.16688/j.zwbh.2024363
L-香芹酮对灰葡萄孢的熏蒸抑菌活性及抑菌机制初探
Preliminary investigation of the fumigation antifungal activity and mechanisms of L-carvone against Botrytis cinerea
摘要
Abstract
To elucidate the fumigation antifungal activity and underlying mechanism of L-carvone,its inhibitory effects on five plant pathogenic fungi(Botrytis cinerea,Sclerotinia sclerotiorum,Sclerotium rolfsii,Fusarium graminearum and Colletotrichum gloeosporioides)were investigated using the mycelial growth rate method.The impact on B.cinerea spore germination was assessed via spore germination assays.Morphological and ultrastructural changes in B.cinerea hyphae following L-carvone fumigation were observed by scanning electron microscopy(SEM)and transmission electron microscopy(TEM),while cell membrane integrity was evaluated using propidium iodide(PI)staining.Moreover,the effects of L-carvone on ergosterol synthesis,extracellular conductivity,and leakage of proteins and nucleic acids were also examined.In vitro assays were also conducted to assess its efficacy in controlling strawberry gray mold.The results showed that L-carvone had significant inhibitory effects on all five plant pathogens,with EC50 values ranging from 5.29 to 18.69 μL/L 72 h after fumigation.The EC50 for B.cinerea spore germination was 11.78 μL/L.B.cinerea hyphae appeared shriveled and distorted,with disorganized organelles after fumigation.PI staining confirmed significant damage to the cell membrane.Ergosterol content was reduced by 27.3％and 43.2％after treated with EC50 and EC90 of L-carvone,respectively.Cytoplasmic leakage resulted in increased relative conductivity,as well as elevated protein and nucleic acid content in the mycelial exudate.EC90 treatment of L-carvone achieved 85.43％therapeutic and 90.31％protective control efficacy against strawberry gray mold.In conclusion,L-carvone inhibits B.cinerea primarily by disrupting cell membrane integrity and permeability,offering a promising strategy for developing plant-derived fumigant fungicides.关键词
L-香芹酮/熏蒸抑菌活性/灰葡萄孢/抑菌机制Key words
L-carvone/fumigation antifungal activity/Botrytis cinerea/mechanism of action分类
农业科技引用本文复制引用
智亚楠,尤伟晨,李芸,李宇洁,王春生,陈利军..L-香芹酮对灰葡萄孢的熏蒸抑菌活性及抑菌机制初探[J].植物保护,2025,51(4):63-71,9.基金项目
河南省自然科学基金(232300420030) （232300420030）
河南省高等学校重点科研项目(25B210017) （25B210017）
信阳农林学院作物绿色防控与品质调控科技创新团队(XNKJTD-007) （XNKJTD-007）
