植物保护2025，Vol.51Issue(4)：81-87,7.DOI:10.16688/j.zwbh.2024472
芜菁花叶病毒广西大化菜心分离物全基因组序列特征分析
Complete genome sequence analysis of turnip mosaic virus isolate from Brassica rapa var.chinensis in Dahua,Guangxi,China
摘要
Abstract
To determine the viral pathogen responsible for mosaic and mottling symptoms observed on Brassica rapa var.chinensis in Dahua,Guangxi,China,four samples with typical symptoms were collected for molecular analysis.Reverse transcription-polymerase chain reaction(RT-PCR),DNA fragment cloning,rapid amplification of cDNA ends(RACE)and phylogenetic analysis were performed.Using universal degenerate primers targeting Potyvirus species,PCR amplification yielded expected products from all four samples.Subsequent cloning and sequencing revealed high nucleotide identity with turnip mosaic virus(TuMV),confirming infection by TuMV.Full-length genome sequencing of a representative isolate was achieved using seven specific primer pairs along with 5′ and 3′ RACE primers.The complete genome was 9 834 nucleotides in length.BLAST analysis showed that the sequence shared 77.8％—96.06％nucleotide identity with other TuMV isolates in GenBank,with the highest similarity(96.06％)to isolate TuMV_CHN229 from Anning,Yunnan(GenBank accession:LC537500.1).This isolate was designated TuMV_GXdh1(GenBank accession:PP079014).Phylogenetic analysis based on the complete genome showed that TuMV_GXdh1 clustered within the world-B group,forming a subgroup with the Yunnan TuMV_CHN229 and Zhejiang TuMV_CHZH33A isolates(GenBank accession:LC537526.1),indicating a close evolutionary relationship.This study represents the first report of a full genome sequence of TuMV from Guangxi,providing new insights into its molecular characteristics and phylogenetic placement.关键词
广西/菜心/芜菁花叶病毒/全基因组序列/world-B组Key words
Guangxi/Brassica rapa var.chinensis/turnip mosaic virus/complete genome/world-B group分类
农业科技引用本文复制引用
莫翠萍,李战彪,陈锦清,李祐聪,谢慧婷,秦碧霞,崔丽贤,李飞飞,汤亚飞,蔡健和..芜菁花叶病毒广西大化菜心分离物全基因组序列特征分析[J].植物保护,2025,51(4):81-87,7.基金项目
广西自然科学基金(2020GXNSFAA259003) （2020GXNSFAA259003）
广西农业科学院基本科研业务专项(桂农科 2021YT071,桂农科 2024ZX08) （桂农科 2021YT071,桂农科 2024ZX08）
