植物遗传资源学报2025，Vol.26Issue(8)：1574-1586,13.DOI:10.13430/j.cnki.jpgr.20241209001
一种新型水稻穗叶类病斑突变体pls1的基因克隆和功能分析
Gene Cloning and Functional Analysis of a New Type of Panicle and Leaf Spot Mutant pls1 in Rice
摘要
Abstract
Rice lesion mimic mutants play an important role in studying programmed cell death and broad-spectrum disease resistance.Previously reported rice lesion mimics mainly occur on leaves and occasionally on glumes.In this study,we report a novel panicle and leaf spot mutant,pls1(Panicle and leaf spot 1),which exhibits reddish-brown lesions starting from the three-leaf stage.The lesions expand with developmental progression and spread to other organs.Unlike previously reported lesion mimic mutants,pls1 develops reddish-brown spots on panicle branches and glumes after heading,leading to severe panicle withering at maturity and significant yield reduction,representing a new type of rice lesion mimic mutant.Map-based cloning combined with whole-genome resequencing revealed a large 173403 bp deletion in the pls1 mutant,resulting in the loss of seven genes and the promoter region of one gene.Among these eight genes,four encode prolamin proteins,while the other three exhibit low expression levels in leaves and panicles.Only Os12g0268000 shows relatively higher expression in leaves and panicles compared to other organs.These results suggest that PLS1 corresponds to Os12g0268000,and functional annotation reveals that it encodes a tryptamine 5-hydroxylase.The pls1 mutant exhibited excessive accumulation of reactive oxygen species,hydrogen peroxide,and superoxide anions,along with increased activity of antioxidant enzymes,including superoxide dismutase,ascorbate peroxidase,catalase,and glutathione reductase.These changes triggered programmed cell death and chloroplast degradation,reducing photosynthetic capacity.Melatonin plays a crucial role in plant salt tolerance.Further functional analysis showed that the loss of PLS1 suppressed the expression of melatonin biosynthesis-related genes(OsTDC1,OsTDC3,OsSNAT1,OsASMT1,and OsCOMT),leading to reduced salt tolerance in pls1.In summary,pls1 is a novel type of rice lesion mimic mutant that will provide new germplasm for studying rice lesion mimics.The salt tolerance analysis reveals a new function of tryptamine 5-hydroxylase,offering insights into its role in programmed cell death and salt stress resistance.关键词
水稻/穗叶类病斑/pls1/褪黑色素/耐盐性Key words
rice/panicle and leaf spot/pls1/melanin/salt tolerance引用本文复制引用
韩沛洁,李飞,叶春,盛怡乐,孙红正,李俊周,杜彦修..一种新型水稻穗叶类病斑突变体pls1的基因克隆和功能分析[J].植物遗传资源学报,2025,26(8):1574-1586,13.基金项目
河南省自然科学基金(232300421156) （232300421156）
河南省科技攻关项目(242102111137) （242102111137）
河南省高等学校重点科研项目(23A210027) （23A210027）
河南省现代农业产业技术体系项目(HARS-22-03-G3) （HARS-22-03-G3）
河南省重点研发专项(231111110500)Natural Science Foundation of Henan(232300421156) （231111110500）
Henan Science and Technology Research Project(242102111137) （242102111137）
Key Scientifc Research Projects of Colleges and Universities in Henan Province(23A210027) （23A210027）
Modern Agricultural Industry Technology System Projects of Henan(HARS-22-03-G3) （HARS-22-03-G3）
Key R&D projects of Henan(231111110500) （231111110500）
