植物遗传资源学报2025，Vol.26Issue(8)：1587-1596,10.DOI:10.13430/j.cnki.jpgr.20250107001
玉米卷叶突变体swl5的基因定位及功能分析
Gene Mapping and Functional Analysis of the Leaf Rolling Mutant swl5 in Maize
摘要
Abstract
Drought represents a significant constraint affecting maize growth and development.Identifying drought-resistant genes is vital for genetic improvement of drought resistance in maize.In this study,the leaf rolling mutant swl5(sensitive to water loss 5)was identified from the EMS-mutagenized mutant library of the inbred line RP125.The swl5 mutant exhibited distinct leaf wilting and rolling phenotype under field drought stress.Map-based cloning localized the genetic locus to a physical region of 280 kb on chromosome 5,containing nine predicted coding genes.In this interval,a C to T mutation occurred at the 748th nucleotide of the GRMZM2G002260 gene,resulting in a premature stop codon.GRMZM2G002260 corresponds to the previously reported ZmWI5,encoding an endo-1,4-xylanase of the GH10.To further validate gene function,the wi5 mutant allele of ZmWI5 was obtained.The F,plants derived from the cross of swl5 and wi5 mutant displayed leaf wilting and rolling phenotypes to both parental mutants.Under drought stress,the swl5 mutant showed significantly reduced photosynthetic rate,stomatal conductance,and transpiration rate compared to the wild type RP125,while displaying elevated leaf temperature and increased accumulation of H2O2 and O2-.Expression of genes involved in cellulose synthesis(CESA5)and xylan synthesis(DUF579,GT47,IRX9,IRX9H-1,IRX9H-2,GUX1,TBL33)was significantly down-regulated in swl5 under both well-watered and drought stress.In summary,swl5 is a new allelic mutation of the ZmWI5 gene.The swl5 mutant exhibits a drought sensitive phenotype,and the expression of genes related to xylan synthesis is significantly reduced in the mutant.关键词
玉米/卷叶突变体/基因定位/干旱Key words
maize/rolled-leaf mutant/gene mapping/drought引用本文复制引用
张莹莹,李健,秦阳,李瑞,张志明,邹华文,郑军,王逸茹..玉米卷叶突变体swl5的基因定位及功能分析[J].植物遗传资源学报,2025,26(8):1587-1596,10.基金项目
国家重点研发项目(2023YFD1200500)The National Key R&D Program of China(2023YFD1200500) （2023YFD1200500）
