植物遗传资源学报2025，Vol.26Issue(8)：1597-1605,9.DOI:10.13430/j.cnki.jpgr.20241126002
苦瓜亚种间遗传图谱构建及果实相关性状QTL定位
Inter-subspecies Genetic Map Construction of Bitter Gourd(Momordica charantia L.)and QTL Mapping for Fruit-related Traits
摘要
Abstract
Bitter gourd(Momordica charantia L.)is a traditional melon vegetable in China,but its cultivated varieties suffer from a narrow genetic background,necessitating the expansion of genetic diversity through distance hybridization with wild germplasm.In this study,an F2 population was generated from a cross between the small-fruited wild subspecies inbred line NJ and the common subspecies inbred line Tan.QTL mapping was performed for five fruit-related traits(fruit length,fruit diameter,fruit shape index,fruit shape,and fruit weight)using a bin marker genetic map through genotyping-by-sequencing,with the MQM(Multiple-QTL model)method.Significant correlations were observed among most traits,except between fruit diameter and fruit shape,as well as between fruit shape index and fruit weight.1563 of 1921 bin markers were integrated into an inter-subspecies genetic map,spanning 1556.91 cM across 11 chromosomes,with an average genetic interval of 1.00 cM.Comparative analysis between the genetic and physical maps revealed approximately 11 Mb inversion on chromosome MC08 between the two subspecies.A total of seven QTL exceeding the LOD threshold were identified,including two for fruit length(qFL5.1,qFL7.1),two for fruit shape index(qFSI1.1,qFSI4.1),two for fruit weight(qFW5.1,qFW6.1),and one for fruit shape(qFS5.1).Notably,qFL5.1,qFS5.1 and qFW5.1 were mapped to a consensus major-effect QTL interval.The findings of this study contribute to our understanding of the genomic variation between the two subspecies of bitter gourd and provide the foundation for inter-subspecies germplasm innovation and gene mining of fruit-related traits.关键词
苦瓜亚种/遗传图谱/果实相关性状/QTLKey words
bitter gourd subspecies/genetic map/fruit-related traits/QTL引用本文复制引用
李炯,徐彬其,梁梓兴,曹振强,侯冰冰,洪宇,程蛟文,胡开林,崔竣杰..苦瓜亚种间遗传图谱构建及果实相关性状QTL定位[J].植物遗传资源学报,2025,26(8):1597-1605,9.基金项目
广州市科技计划(2024A04J5656) （2024A04J5656）
广东省省级科技计划(2022B0202160015) （2022B0202160015）
广东省乡村振兴战略专项资金-种业振兴(2022-NPY-00-027)Guangzhou Science and Technology Plan(2024A04J5656) （2022-NPY-00-027）
Guangdong Provincial Science and Technology Plan(2022B0202160015) （2022B0202160015）
Guangdong Provincial Rural Revitalization Strategy Special Fund-Seed Industry Revitalization(2022-NPY-00-027) （2022-NPY-00-027）
