作物杂志Issue(4)：19-28,10.DOI:10.16035/j.issn.1001-7283.2025.04.003
玉米ZmCaM1基因的克隆和表达分析
Cloning and Expression Analysis of Maize ZmCaM1 Gene
摘要
Abstract
Calmodulin(CaM)plays an important role in the response of plants to environmental stress.A CaM gene with a high expression level in the root system after drought stress was cloned based on the transcriptome data of the maize(Zea mays L.)inbred line B73,and bioinformatics analysis was conducted.The results showed that the enconding sequence of ZmCaM1 is 450 bp,encoding 149 amino acids.The ZmCaM1 protein lacked a signal peptide and exhibited hydrophilic properties.The EF-hand domain of the ZmCaM1 protein was highly conserved among plants,such as sorghum(Sorghum bicolor L.)and rice(Oryza sativa L.),and presented similar secondary and tertiary structures.There were several cis-acting elements related to hormones and abiotic stress in ZmCaM1 promoter.In addition,we predicted that ZmCaM1 protein could bind the promoter of RAD4 according to bioinformatics methods,and confirmed above results by yeast two-hybrid.By optimizing the induction conditions for the GST-ZmCaM1 protein,we found that the best expression was achieved under 0.1 mmol/L IPTG,and the GST-ZmCaM1 protein was purified via gel affinity chromatography.Expression analysis showed that the expression level of ZmCaM1 gene was higher in roots and leaves of maize at emergence stage.After drought stress,ZmCaM1 was up regulated in roots and leaves,and it was down regulated in roots and leaves under waterlogging stress.The association between the ZmCaM1 gene with the growth and development of maize as well as the response to abiotic stress were verified.关键词
玉米/钙调蛋白/非生物胁迫/生物信息学/表达分析Key words
Maize/Calmodulin/Abiotic stress/Bioinformatics/Expression analysis引用本文复制引用
贺新春,杜何为,黄敏..玉米ZmCaM1基因的克隆和表达分析[J].作物杂志,2025,(4):19-28,10.基金项目
国家自然科学基金(31771801) （31771801）
湖北省自然科学基金面上项目(2022CFB183) （2022CFB183）
湖北省自然科学基金创新群体项目(2022CFA030) （2022CFA030）
