作物杂志Issue(4)：29-40,12.DOI:10.16035/j.issn.1001-7283.2025.04.004
基于SSR技术的马铃薯种质资源遗传多样性分析
Genetic Diversity Analysis of Potato Germplasm Resources Based on SSR Technology
摘要
Abstract
To clarify the genetic relationship of potato germplasm resources,SSR markers were used to analyze the genetic diversity of 63 potato germplasm resources.The results showed that 164 polymorphic bands were amplified,including 145 polymorphic loci,the average number of bands was 5.13.The number of effective alleles,Nei's genetic diversity index and Shannon index were 1.7139,0.3927 and 0.5632,respectively,and the genetic distance ranged from 0.12 to 0.71.The tested materials could be divided into five groups at the genetic distance value of 0.180,and the clustering results were highly consistent with their geographical origins.The results of PCA for 63 germplasm resources showed that the total variation of the three principal components was 45.04%,and were consistent with the clustering results.DNA fingerprints of 63 germplasm resources were constructed using SSR primers STL001,S192 and S118,and QR code molecular identity cards were established.This research lays a foundation for potato resource utilization and variety improvement.关键词
马铃薯/种质资源/SSR分子标记/遗传多样性/分子身份证Key words
Potato/Germplasm resources/SSR molecular marker/Genetic diversity/Molecular ID card引用本文复制引用
王丹,刘宇飞,张丹,张志成,韩海霞,韩娜,王春勇,王正,潘孟洋,李强,王懿茜..基于SSR技术的马铃薯种质资源遗传多样性分析[J].作物杂志,2025,(4):29-40,12.基金项目
内蒙古自然科学基金(2023QN03056,2021BS03010) （2023QN03056,2021BS03010）
内蒙古农牧业青年创新基金(2023QNJJN13) （2023QNJJN13）
集宁师范学院博士基金(jsbsjj1707,jsbsjj2309) （jsbsjj1707,jsbsjj2309）
