作物杂志Issue(4)：41-48,8.DOI:10.16035/j.issn.1001-7283.2025.04.005
du1新等位变异的克隆及其分子标记的开发
Cloning of the du1 Novel Allelic Variant and the Development of Its Molecular Markers
摘要
Abstract
The"Shuang Tian Nuo"maize is a new type of maize germplasm,whose endosperm characteristics are jointly controlled by the waxy gene and the sweet gene.However,the sweet regulation gene has not yet been located and cloned.The results showed that the soluble sugar content of the naturally air-dried grains of"Shuang Tian Nuo"maize XT was 62.82 mg/g,significantly higher than the 19.32 mg/g of ordinary maize,but lower than the 110.9 mg/g of sugary(su1)maize.Using BSR-seq technology and based on the wrinkled grain phenotype,the sweet regulatory gene of XT maize was mapped to the 24-68 Mb interval on chromosome 10.By comparing the full-length DNA sequence of the candidate Du1gene in XT maize and ordinary maize B73,a 5839 bp Gypsy-like LTR retrotransposon insertion was found after the 1455 bp position in the third exon of the du1 gene in XT maize.cDNA sequence analysis revealed that the transposon insertion sequence in the du1 gene of XT maize was transcribed in large segments,resulting in abnormal transcripts.So it was determined as the key gene for sugary quality regulation.This gene is a new allelic variant of the du1 gene,and the KASP molecular marker developed for its mutation site can efficiently distinguish betweendu1du1,du1Du1,and Du1Du1 three genotypes.关键词
玉米/du1新等位变异/BSR-seq/反转座子插入/KASP分子标记Key words
Maize/New du1 allelic variation/BSR-seq/Retrotransposon insertion/KASP molecular marker引用本文复制引用
史亚兴,骆美洁,刘俊玲,朱贵川,赫忠友,刘辉,樊艳丽,徐丽,卢柏山,赵久然..du1新等位变异的克隆及其分子标记的开发[J].作物杂志,2025,(4):41-48,8.基金项目
北京市农林科学院创新能力建设专项(KJCX20240408) （KJCX20240408）
