作物杂志Issue(4)：75-79,5.DOI:10.16035/j.issn.1001-7283.2025.04.009
121份自选小麦新品种(系)高分子量麦谷蛋白亚基组成分析
Composition Analysis of High Molecular Weight Glutenin Subunits in 121 Wheat Varieties(Lines)
摘要
Abstract
The high molecular weight glutenin subunits(HMW-GS)of 121 self-selected high generation wheat varieties(lines)were identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE),and their subunit types and combinations were analyzed.The results showed that there were nine subunit types in 121 wheat varieties(lines).Among them,there were two types of Glu-A1 sites,including 1 and N.There were four kinds of Glu-B1 sites,including 7,7+8,7+9 and 13+16.There were three kinds of Glu-D1 sites,including 5+10,4+12 and 2+12.There were ten subunit combinations in 121 wheat varieties(lines),among which 1/7+8/5+10 subunit combinations were the most with a frequency of 52.90%,followed by 1/7+9/5+10,1/7+8/2+12,1/7+9/2+12,and their frequency was 23.14%,9.10%,5.79%,respectively.The frequency of other subunit combinations was less than 5%.The quality scores of all varieties(lines)ranged from 5-10.The results showed that there were abundant polymorphisms of HMW-GS in all the 121 new wheat varieties(lines).The high-quality subunit 5+10 had the highest frequency,rare subunit 13+16 was found,and no high-quality subunits 2*,14+15 and 17+18 were found.关键词
小麦/品种(系)/高分子量麦谷蛋白亚基/亚基组成Key words
Wheat/Varieties(lines)/High molecular weight glutenin subunits/Subunit composition引用本文复制引用
窦阳,陈君燚,任淑敏,康国章,王黎明..121份自选小麦新品种(系)高分子量麦谷蛋白亚基组成分析[J].作物杂志,2025,(4):75-79,5.基金项目
国家自然科学基金类―河南省联合基金项目(U1904108,U1304318) （U1904108,U1304318）
河南省科技攻关(农业领域)研究项目(202102110022) （农业领域）
省部共建小麦玉米作物学国家重点实验室2021年度开放课题项目(SKL2021KF04) （SKL2021KF04）
