阳荷多糖提取工艺优化及其生物活性研究OA北大核心

To expand the utilization of Zingiber striolatum(a characteristic plant resource)and enhance the value-added potential of its products,Zingiber striolatum polysaccharides(ZSP)were extracted from fresh Zingiber striolatum using aqueous extraction followed by ethanol precipitation.The extraction process was optimized via single-factor and response surface experiments,and the biological activities of ZSP were evaluated,including emulsifying property,antibacterial activity,heavy metal adsorption capacity,antioxidant activity,and hypoglycemic potential.The optimal extraction parameters were:particle size 80 mesh,solid-liquid ratio 1∶65,extraction temperature 75℃,extraction time 96 min,and ethanol volume 400 mL.Under these conditions,the ZSP yield was(3.89±0.01)%.The emulsifying property of ZSP increased with higher mass concentration,reaching a maximum of(76.14±1.33)%at 15 mg/mL.ZSP exhibited antibacterial activity against Escherichia coli,with inhibition zone diameter increasing at higher concentrations;at 50 mg/mL,the inhibition zone diameter was(2.35±0.02)cm.The adsorption capacities of ZSP for Cu2+,Cd2+and Pb2+increased at higher mass concentrations,reaching(17.26±0.41)mg/g,(21.19±0.33)mg/g,and(27.64±0.61)mg/g at 1.0 g/L,respectively.The scavenging activities of ZSP against ABTS+,DPPH,and·OH radicals increased with higher mass concentration,with scavenging rates of(52.10±0.01)%,(60.40±1.95)%,and(22.20±0.02)%at 3 mg/mL,respectively.The inhibitory activity of ZSP against α-glucosidase increased with higher mass concentration,reaching a maximum of(38.00±2.00)%at 1.0 mg/mL.Collectively,ZSP demonstrated significant potential in heavy metal adsorption and hypoglycemic activity,suggesting its promise for developing edible materials for heavy metal removal and antidiabetic functional products.