轻工学报2025，Vol.40Issue(4)：20-29,10.DOI:10.12187/2025.04.003
硒代蛋氨酸对珍珠龙胆石斑鱼肌原纤维蛋白体外消化特性的影响
Effects of selenomethionine on the in vitro digestibility of myofibrillar proteins in pearl gentian grouper
摘要
Abstract
In this study,the effects of selenomethionine on the in vitro digestibility of myofibrillar protein(MP)in pearl gentian grouper were investigated using a static in vitro digestion model to simulate gastricointestinal digestion.The results showed that after simulated gastricointestinal digestion,the selenium content in the high-level selenomethionine group(Y2 group)was 0.130 mg/kg(gastric)and 0.072 mg/kg(intestinal),significantly higher than that in the control group(C group)and low-level selenomethionine group(Y1 group)(P<0.05).Specifically,the carbonyl content(2.02 nmol/mg and 2.59 nmol/mg),dityrosine content(3962 a.u.and 4062 a.u.),thiobarbituric acid(TBA)value(0.31 mg/kg and 0.32 mg/kg),and particle size(365 nm and 357 nm)in the Y2 group were significantly lower than those in the C and Y1 group(P<0.05).Additionally,the Y2 group exhibited higher UV absorption peak intensities and endogenous fluorescence intensities,indicating that high-level selenomethionine delayed MP oxidation and denaturation,thereby better maintaining the structural stability of MP during in vitro digestion.The in vitro digestibility(62.19%and 67.25%)and degree of hydrolysis(0.31 mmol/g and 0.34 mmol/g)in the Y1 group were significantly higher than those in the C and Y2 group(P<0.05).In conclusion,supplementation with selenomethionine not only enhanced the antioxidant capacity of MP but also improved their in vitro digestibility,suggesting that selenomethionine has potential applications in enhancing the nutritional quality and stability of fish proteins.关键词
珍珠龙胆石斑鱼/硒代蛋氨酸/肌原纤维蛋白/体外消化特性/模拟胃肠道消化Key words
pearl gentian grouper/selenomethionine/myofibrillar protein/in vitro digestibility/simulated gastricointestinal digestion分类
轻工纺织引用本文复制引用
徐愉聪,何圣琪,栾宏伟,步营,朱文慧,励建荣,李学鹏..硒代蛋氨酸对珍珠龙胆石斑鱼肌原纤维蛋白体外消化特性的影响[J].轻工学报,2025,40(4):20-29,10.基金项目
辽宁省教育厅基本科研项目面上项目(LJKMZ20221486) （LJKMZ20221486）
辽宁省自然科学基金面上项目(2023-MS-297) （2023-MS-297）
渤海大学海洋研究院开放课题资助项目(BDHYYJY2024012) （BDHYYJY2024012）
