长春中医药大学学报2025，Vol.41Issue(8)：866-870,5.DOI:10.13463/j.cnki.cczyy.2025.08.010
温阳督针疗法对缺血缺氧型脑瘫幼鼠干预效果及机制研究
Study on the intervention effect and mechanism of Wenyang Duzhen therapy on ischemic and hypoxic cerebral palsy in young mice
摘要
Abstract
Objective To explore the intervention effect and potential mechanism of Wenyang Duzhen therapy on ischemic and hypoxic cerebral palsy in young mice.Methods 35 7-day-old SPF grade SD newborn rats were selected,10 of them were in the sham operation group,and 25 of them were in the hypoxic-ishcemic cerebral palsy model preparation.Finally,5 rats died and 20 rats were successfully modeled.20 young rats with cerebral palsy were divided into the model group and the acupuncture and moxibustion group according to random number table method,with 10 rats in each group.The sham operation group only isolated the common carotid artery without ligation and did not undergo hypoxia treatment.During the treatment,the sham operation group and the model group only used the same grabbing stimulation as the acupuncture and moxibustion group,and the acupuncture and moxibustion group was treated with Wenyang Duzhen therapy,Baihui point was treated with acupuncture,Dazhui point and Zhiyang point were treated with moxibustion for 28 days.After treatment,HE staining was used to observe the pathological changes in hippocampal tissue,TUNEL method was used to detect apoptosis of hippocampal tissue cells,ELISA method was used to detect the levels of interleukin(IL)-1β,IL-6,and IL-10 in serum,and Western blot was used to detect the expression levels of p-JAK2 and p-STAT3 proteins in hippocampal tissue.Results In the model group,a large number of nerve cells in the hippocampal tissue of mice were damaged,shrunk,and deeply stained in cytoplasm;The apoptosis rate increased significantly(P<0.05);And the levels of pro-inflammatory factors of IL-1β and IL-6 in the serum increased(P<0.05),while the level of the anti-inflammatory factor of IL-10 decreased(P<0.05),and the expression levels of p-JAK2 and p-STAT3 proteins in the hippocampal tissue increased(P<0.05).Compared with the model group,the injury of the hippocampal tissue in the acupuncture and moxibustion group was alleviated,the number of apoptotic cells decreased,the apoptosis rate decreased(P<0.05),the levels of IL-1β and IL-6 in the serum decreased(P<0.05),the level of IL-10 increased(P<0.05),and the levels of p-JAK2 and p-STAT3 proteins in the hippocampal tissue decreased(P<0.05).Conclusion Wenyang Duzhen therapy has a certain intervention effect on ischemic and hypoxic cerebral palsy in young mice,and its mechanism may be related to regulating the levels of inflammatory factors,inhibiting cell apoptosis,and regulating the JAK2/STAT3 signaling pathway.关键词
温阳督针疗法/脑瘫/海马组织/炎症因子/细胞凋亡/JAK2/STAT3信号通路Key words
Wenyang Duzhen therapy/cerebral palsy/hippocampal tissue/inflammatory factor/cell apoptosis/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
周楚璇,周钰..温阳督针疗法对缺血缺氧型脑瘫幼鼠干预效果及机制研究[J].长春中医药大学学报,2025,41(8):866-870,5.基金项目
国家自然科学基金项目(82360970) （82360970）
