西部中医药2025，Vol.38Issue(7)：6-11,6.DOI:10.12174/j.issn.2096-9600.2025.07.02
杜仲腰痛丸干预大鼠椎间盘软骨终板细胞的机制研究
Study on the Mechanism of Duzhong Yaotong Pills Intervention for Rat Intervertebral Disc Cartilage Endplate Cells
摘要
Abstract
Objective:To discuss the effects of Duzhong Yaotong pills on rat intervertebral disc cartilage endplate cells.Methods:Fifty rats were randomized into five groups including the control group,the model group,diclofenac sodium(voltaren)group,high and low dosages groups of Duzhong Yaotong pills(n=10 in each group).Rat models suffering lumbar disc herniation(LDH)were duplicated successfully using etiological simulation modelling methods,and they accepted the corresponding therapy in different groups.After collecting the samples of rat intervertebral disc cartilage endplate cells from L6-7,RT-PCR was applied to detect the expressions of the factors related to intervertebral disc degeneration,TGF-β1,Smad2 and Smad4,HOXB4 and COL11A1mRNA in the samples,WB was applied to measure the expressions of the protein related to intervertebral disc degeneration such as TGF-β1,Smad2 and Smad4,HOXB4 and COL11A1 protein,and HE staining to observe the pathological changes of intervertebral disk cartilage.Results:The relative expressions of TGF-β1,Smad2 and Smad4,HOXB4 and COL11A1mRNA were lowered in the model group than blank control group(P<0.01);after treatment with diclofenac sodium,the relative expressions of TGF-β1,COL11A1 and Smad2 mRNA were elevated than the model group(P<0.05);the relative expressions of TGF-β1,Smad2 and Smad4,HOXB4 and COL11A1 mRNA in the intervertebral disc were higher after the treatment with the mixture of Duzhong Yaotong pills than these of model control group(P<0.05);the relative expressions of TGF-β1,Smad2,HOXB4 and COL11A1 mRNA in the high dosage group were higher than these in the low dosage group(P<0.05).In terms of the expressions of the protein,compared with blank control group,the relative expressions of TGF-β1,Smad2,HOXB4 and COL11A1 protein were higher in the model group(P<0.05);after diclofenac sodium treatment,the relative expressions of TGF-β1 and Smad4 were higher than these of the model group(P<0.05);the relative expressions of TGF-β1,Smad2 and Smad4,HOXB4 and COL11A1 protein in the high dose group of the medicine were increased than these of the model group(P<0.05);when low dosage group of the medicine was compared with the model group,the relative expressions of TGF-β1,Smad2 and Smad4,and COL11A1 protein were higher(P<0.05).Conclusion:The mixture of Duzhong Yaotong pills could inhibit the apoptosis of cartilage endplate cells and protect intervertebral disc cartilage endplate cells,and the related molecular mechanism might be related to the regulation of TGF-β1/Smad2/Smad4-HOXB4/COL11A1 signaling pathway.关键词
杜仲腰痛丸/腰椎间盘突出/腰为肾之府/机制Key words
Duzhong Yaotong pills/vertebral herniation/the waist is the seat of the kidney/mechanism分类
医药卫生引用本文复制引用
李家明,张彦军,郭铁峰,王鹏,罗林钊,刘晓雪,马平怡,江朔轩..杜仲腰痛丸干预大鼠椎间盘软骨终板细胞的机制研究[J].西部中医药,2025,38(7):6-11,6.基金项目
基于中医临床研究基地优势病种的中医临床疗效能力提升(20210200111). （20210200111）
