西部中医药2025，Vol.38Issue(7)：18-23,6.DOI:10.12174/j.issn.2096-9600.2025.07.04
基于PI3K/AKT/Foxo1信号通路探讨大黄素防治非酒精性脂肪肝的作用机制
Emodin Prevents and Treats NAFLD via PI3K/AKT/Foxol Signaling Pathway
摘要
Abstract
Objective:To investigate the mechanism of emodin in improving lipid accumulation model with NAFLD induced by oleic acid+palmic acid.Methods:Network pharmacology was utilized to predict emodin in the intervention of the signaling pathway of NAFLD;the in-vitro models of NAFLD were simulated using oleic acid+palmic acid,and intervened with 15 and 30 μM of emodin for 48 hours,to detect the contents of TG,oil red O(ORO)staining was used to assess cell lipid accumulation,WB was applied to measure PI3K,AKT2,pFoxo1/Foxo1 and nuclear pFoxo1/Foxo1,real-time quantitative PCR was utilized to detect the expressions of PI3K,AKT2,Foxo1,microsomal triglyceride transfer protein and apoC-Ⅲ.Results:In total,34 targets were predicted for emodin by network pharmacology,and these targets were significantly enriched in the signaling pathways such as insulin resistance and PI3K/AKT/Foxol.The contents of TG were lowered in the cells after intervened with 15 and 30 μM of emodin;emodin could improve lipid accumulation in BRL cells induced by oleic acid+palmic acid;30 μM of emodin could noticeably upregulate the levels of PI3K and AKT of the model group,and downregulate pFoxo1/Foxo1;30 μM of emodin could lower the expressions of MTTP and apoC-Ⅲ of the model group.Conclusion:Emodin could reduce lipid accumulation possibly through adjusting PI3K/AKT/Foxol.关键词
脂肪性肝,非酒精性/大黄素/甘油三酯/总磷脂酰肌醇3-激酶/蛋白激酶Bβ/叉头框蛋白1/甘油三酯转运蛋白/载脂蛋白CⅢKey words
fatty liver disease,nonalcoholic/emodin/triglyceride/phosphatidylinositol 3-kinase/RAC-beta serine/threonine-protein kinase/forkhead box/triglyceride transport protein/apolipoprotein分类
医药卫生引用本文复制引用
吴海滨,林基伟,宋晓容,程波敏,刘卓超,朱艳萍,谭梅傲..基于PI3K/AKT/Foxo1信号通路探讨大黄素防治非酒精性脂肪肝的作用机制[J].西部中医药,2025,38(7):18-23,6.基金项目
国家自然科学青年基金(82204953) （82204953）
广东省中医药局科研项目(20211325) （20211325）
深圳市卫生健康委员会医防融合中医药组项目(深卫健体改[2019]25号) （深卫健体改[2019]25号）
深圳市中医"治未病"重点专科建设项目 （）
成都中医药大学杏林学者科研提升计划(YYZX2021056) （YYZX2021056）
重庆市自然科学基金—博士后项目(CSTB2022NSCQ-BHX0032). （CSTB2022NSCQ-BHX0032）
