海南医科大学学报2025，Vol.31Issue(15)：1121-1129,9.DOI:10.13210/j.cnki.jhmu.20241108.003
外泌体滴鼻对抑郁小鼠行为的影响及单胺类递质释放水平的改变观察
Observation of the effects of exosome intranasal administration on the behavior of depressed mice and changes in monoamine neurotransmitter release levels
摘要
Abstract
Objective:To investigate the effects of intranasal administration of adipose-derived mesenchymal stem cell exo-somes(ADMSCs-EXO)on depressive behaviors in mice and its underlying mechanisms.Methods:Chronic binding stress was used to develop depression models,with EXO or PBS applied once daily for 8 weeks,and normal control mice were not treated.The depressive behavior was assessed using the suspended tail and forced swim experiment.Immunofluorescence was utilized to observe the brain entry pathway of EXO labeled with PKH26.Following additional behavioral testing,ELISA assay was conduct-ed to detect changes in monoamine neurotransmitters in the hippocampus.Results:After 72 h of intranasal administration of EXO,it reached brain regions such as the hippocampus.After 8 weeks of treatment,the improvement in depressive behavior in mice from the CMS+EXO group was statistically significant(P<0.05).Furthermore,statistically significant increases in the lev-els of 5-hydroxytryptamine,dopamine,and norepinephrine in the hippocampus were observed(P<0.01).Conclusion:Intranasal administration of ADMSCs-EXO can alleviate depressive behavior in mice and elevate monoamine neurotransmitter levels,sug-gesting that intranasal EXO may represent a novel therapeutic strategy for treating depression.关键词
脂肪间充质干细胞/外泌体/抑郁/行为/小鼠/单胺类神经递质Key words
Adipose-derived mesenchymal stem cells/Exosomes/Depression/Behavior/Mice/Monoamine neurotransmit-ters分类
医药卫生引用本文复制引用
田梦思,郑育杰,沈雅婷,郭青云,易西南..外泌体滴鼻对抑郁小鼠行为的影响及单胺类递质释放水平的改变观察[J].海南医科大学学报,2025,31(15):1121-1129,9.基金项目
This study was supported by the National Natural Science Foundation of China(82301632),Social Development Project of Hainan Provincial Department of Science and Technology(ZDYF2024SHFZ137,ZDYF2020145),Open Project Fund of The Key Laboratory of Brain Science Research and Transformation in Tropical Environment of Hainan Province(2022001),and Graduate Innovation Program of Hainan Medical University(HYYB2022A15) 国家自然科学基金资助项目(82301632) （82301632）
海南省科技厅社会发展项目(ZDYF2024SHFZ137,ZDYF2020145) （ZDYF2024SHFZ137,ZDYF2020145）
海南省热带环境脑科学研究与转化重点实验室开放项目基金(2022001) （2022001）
海南医科大学研究生创新项目(HYYB2022A15) （HYYB2022A15）
