海南医科大学学报2025，Vol.31Issue(15)：1130-1140,11.DOI:10.13210/j.cnki.jhmu.20241021.001
牡荆素对多囊卵巢综合征大鼠卵巢纤维化的改善作用
Ameliorating effect of Vitexin on ovarian fibrosis in rats with polycystic ovary syndrome
摘要
Abstract
Objective:To investigate the ameliorating effect of Vitexin on ovarian fibrosis in rats with polycystic ovary syn-drome(PCOS).Methods:A total of 40 5-week-old female SD rats were randomly divided into a normal(control)group,a mod-el(PCOS)group,a metformin(Met)group,a Vitexin low dose(Vitexin-L)group,and a Vitexin high dose(Vitexin-H)group.In addition to the control group,each group was given a daily dose of latrozole(1 mg/kg);In addition,the Met group received metformin(256 mg/kg)every day;the Vitexin-L group was given a daily dose of Vitexin(20 mg/kg);and the Vitexin-H group was given a daily of dose of Vitexin(40 mg/kg).Pap staining was used to detect the vaginal smears of rats in each group,and the estrous cycle was counted.After 28 d of continuous gavage,the body weight of rats was measured,the ovarian morphological changes and lipid metabolism levels of rats were observed by H&E and Masson's staining,serum sex hormone levels and related oxidative stress factor levels were detected by enzyme-linked immunosorbent assay,and the protein expression of fibrotic factors in rat ovarian tissues was detected by Western blot.Results:Vitexin significantly reduced the body weight of PCOS rats(P<0.01),regulated the levels of serum sex hormones testosterone,estradiol,luteinizing hormone,and follicle-stimulating hormone,signifi-cantly reduced the levels of LDL cholesterol,triglycerides,total cholesterol,and malondialdehyde in PCOS rats,enhanced the ac-tivity of antioxidant enzymes,and reduced the expression of fibrotic protein in ovarian tissue.Conclusion:Vitexin administration regulates the level of sex hormones,lipid metabolism,and oxidative stress in PCOS rats,and then improves the fibrosis of the ovaries of rats.关键词
多囊卵巢综合征/牡荆素/氧化应激/纤维化Key words
Polycystic ovary syndrome/Vitexin/Oxidative stress/Fibrosis分类
医药卫生引用本文复制引用
宋子纯,闫传智,徐婕径,王可盈,宋家乐,周燕园..牡荆素对多囊卵巢综合征大鼠卵巢纤维化的改善作用[J].海南医科大学学报,2025,31(15):1130-1140,11.基金项目
This study was supported by the National Natural Science Foundation of China(82360854),Natural Science Foundation Project of Guangxi Province(2022GXNSFAA035603) （82360854）
Central Government Guides Local Science and Technology Development Special Fund Projects(Guike ZY22096025),Guangxi Key Laboratory of Drug Molecule Discovery and Druggability Optimization Open Project Funding(GKLPMDDO-2022-C01),and Guilin Science and Technology Program(20220139-8-2) 国家自然科学基金资助项目(82360854) （Guike ZY22096025）
广西自然科学基金资助项目(2022GXNSFAA035603) （2022GXNSFAA035603）
中央引导地方科技发展资金专项(桂科ZY22096025) （桂科ZY22096025）
广西药物分子发现与成药性优化重点实验室开放课题资助项目(GKLPMDDO-2022-C01) （GKLPMDDO-2022-C01）
桂林市科技计划资助项目(20220139-8-2) （20220139-8-2）
