海南医科大学学报2025，Vol.31Issue(15)：1172-1181,10.DOI:10.13210/j.cnki.jhmu.20241205.001
肠安康治疗溃疡性结肠炎的作用机制研究
Effect of ChangAnKang on ulcerative colitis based on network pharmacology
摘要
Abstract
Objective:To investigate the therapeutic effect and mechanism of ChangAnKang in treating ulcerative colitis.Methods:Using TCMSP database,we identified the active components of ChangAnKang.Using Pubchem,Swisstarget forecast and Uniprot database,we got the potential action targets of TCM active ingredients.Target genes of ulcerative colitis were ac-quired by Genecards,DisGeNET and OMIM.Using STRING and Cytoscape 3.9.1,the protein-protein interaction network for treating ulcerative colitis was constructed,and then analyzed with GO and KEGG.Finally,by using Autodock,the binding proba-bility of the core chemical composition with the core target was predicted.A model of ulcerative colitis was established to detect the effect of intestinal health on ulcerative colitis by HE staining and to verify the prediction results of the network pharmacological signaling pathway.Results:A total of 93 active ingredients,227 targets and 2 545 disease-related targets were identified in this study,and the"drug-active ingredient target"network had 333 nodes and 1 078 edges.The enrichment of GO and KEGG was used to identify the first 20 biological functions and signaling pathways.It was found that there was good binding probability of the core chemical composition with the core target.Conclusion:ChangAnKang effectively improves the morphological structure of ulcer-ative colitis.ChangAnKang may regulate cancer pathway,hepatitis B,MAPK signaling pathway,PI3K-AKT signaling pathway,human cytomegalovirus infection,chemical carcinogenesis-receptor-initiated,Toll-like receptor signaling pathway through the main active ingredients,quercetin,prickly aristolochicin,kaempferol and other signaling pathways,exerting a therapeutic effect on ulcerative colitis.关键词
溃疡性结肠炎/信号通路/炎症因子/网络药理学/分子对接验证Key words
Ulcerative colitis/Signaling pathways/Inflammatory factors/Network pharmacology/Molecular docking vali-dation分类
医药卫生引用本文复制引用
陆振华,张国帅,范明明,张琳,吕波,邱冰,李阳光,李慧,王欣..肠安康治疗溃疡性结肠炎的作用机制研究[J].海南医科大学学报,2025,31(15):1172-1181,10.基金项目
This study was supported by the Heilongjiang Province Traditional Chinese Medicine Research Project(ZHY2020-050) 黑龙江省中医药科研项目(ZHY2020-050) （ZHY2020-050）
