河北医学2025，Vol.31Issue(7)：1063-1068,6.DOI:10.3969/j.issn.1006-6233.2025.07.02
吉马酮调节RhoA/ROCK信号通路对结直肠癌细胞恶性生物学行为的影响
Effect of Gimadione on Malignant Biological Behaviors of Colorectal Cancer Cells by Regulating the RhoA/ROCK Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of germacrone on the malignant biological behavior of colorectal cancer(CRC)cells based on the RhoA/ROCK signaling pathway.Methods:CRC SW480 cells were grouped into control group,L-germacrone group,M-germacrone group,H-germacrone group,Y-27632 group,and H-germacrone+LPA group.CCK-8 assay kit,flow cytometry,scratch assay,Transwell method,and Western blot were used to detect the proliferation activity,apoptosis,migration,invasion abili-ties,and RhoA/ROCK signaling pathway protein expression in SW480 cells.Results:Compared with the control group in terms of OD value,apoptosis rate,scratch healing rate,invasive cell number,RhoA,and ROCK protein expression,the L-gemone group,the M-gemone group,the H-Guimarone group,Y-27632 group cell OD values,scratch healing rate,invasive cell number,RhoA,ROCK protein expression was signif-icantly decreased(P<0.05)and apoptosis rate was significantly increased(P<0.05);Compared with the H-gemone group,the cell OD value(P<0.001),scratch healing rate(P<0.001),number of invasive cells(P<0.001),RhoA(P<0.001),and ROCK(P<0.001)protein expression were significantly higher(P<0.05)in the H-gimenone+LPA group,and the apoptosis rate(P<0.001)was significantly reduced(P<0.05).Conclusion:The effect of gimarone on the malignant biological behaviour of CRC cells may be achieved by inhibiting the RhoA/ROCK signalling pathway.关键词
吉马酮/RhoA/ROCK信号通路/结直肠癌/增殖/凋亡Key words
Germacrone/RhoA/ROCK signaling pathway/Colorectal cancer/Proliferation/Apoptosis引用本文复制引用
师红利,王莉,胡帅,张慧慧,袁丽芳,张玲玥..吉马酮调节RhoA/ROCK信号通路对结直肠癌细胞恶性生物学行为的影响[J].河北医学,2025,31(7):1063-1068,6.基金项目
山西省临汾市软科学研究项目,(编号:2310) （编号:2310）
