河北医学2025，Vol.31Issue(7)：1068-1075,8.DOI:10.3969/j.issn.1006-6233.2025.07.03
基于PI3K/AKT通路探讨肺岩宁方对肺腺癌细胞奥希替尼耐药的逆转作用
Exploring the Reversal Effect of Feiyanning Formula on the Resistance of Lung Adenocarcinoma Cells to Osimertinib Based on PI3K/AKT Pathway
摘要
Abstract
Objective:To discuss the reversal effect of Feiyanning formula on the resistance of lung ade-nocarcinoma cells to osimertinib(AZD9291)based on the phosphatidylinositol 3-kinase(PI3K)/protein ki-nase B(AKT)pathway.Methods:Human lung adenocarcinoma cell line H1975 was cultured in vitro,and AZD9291 resistant cell line(H1975/AZD)was established.The effect of different concentrations of Feiyan-ning formula on the activity of H1975/AZD cells was measured by MTT assay.H1975/AZD cells were grouped into H1975/AZD group,low-dose Feiyanning formula group(10g/L),high-dose Feiyanning formu-la group(15g/L),high-dose Feiyanning formula+PI3K activator(740Y-P)group(15g/L Feiyanning for-mula+30 μmoL/L 740Y-P),and cells without established drug resistance were marked as the control group.CCK8 method and clone formation assay were performed to detect the proliferation ability of H1975 cells.Flow cytometry was used to measure apoptosis of H1975 cells.The scratch healing experiment was performed to de-tect the migration ability of H1975 cells in each group.Transwell experiment was performed to measure the in-vasion of H1975 cells in each group.QRT-PCR was applied to measure the mRNA expression levels of Bcl-2,Bax,PI3K,and AKT in H1975 cells.Western blot was applied to detect Bcl-2,Bax,and PI3K/AKT pathway related proteins in H1975 cells.Results:Compared with the control group,there was no statistically significant difference in various behavioral indicators of cells in the H1975/AZD group(P>0.05).For the H1975/AZD group,the low and high dose Feiyanning formula groups showed a decrease in cell survival rate,clone formation number,scratch healing rate,number of invasive cells,Bcl-2 mRNA and protein,and an in-crease in cell apoptosis rate,Bax,PI3K,AKT mRNAs,and Bax,p-PI3K,p-AKT proteins(P<0.05).For the high-dose Feiyanning formula group,the trends of the above indicators in the cells of the high-dose Feiy-anning formula+740Y-P group were reversed(P<0.05).Conclusion:Feiyanning formula can reverse the resistance of lung adenocarcinoma cells to osimertinib by inhibiting the activation of PI3K/AKT pathway.关键词
肺腺癌/肺岩宁方/奥希替尼/耐药性/磷脂酰肌醇3激酶/蛋白激酶B通路Key words
Lung adenocarcinoma/Feiyanning formula/Osimertinib/Drug resistance/Phosphatidylinositol 3-kinase/protein kinase B pathway引用本文复制引用
白月琴,刘畅,赵艳莉,张梦馨,樊佳鑫..基于PI3K/AKT通路探讨肺岩宁方对肺腺癌细胞奥希替尼耐药的逆转作用[J].河北医学,2025,31(7):1068-1075,8.基金项目
陕西省教育厅专项科研计划项目,(编号:20JK0600) （编号:20JK0600）
陕西中医药大学学科建设基金项目,(编号:2020XK05) （编号:2020XK05）
