河北医学2025，Vol.31Issue(7)：1082-1087,6.DOI:10.3969/j.issn.1006-6233.2025.07.05
PNMA5在胃癌组织中的表达及其与胃癌细胞恶性生物学行为的关系研究
The Expression of PNMA 5 in Gastric Cancer Tissues and its Relationship with the Malignant Biological Behaviors of Gastric Cancer Cells
摘要
Abstract
Objective:To investigate the expression of paraneoplastic antigen Ma family member 5(PN-MA5)in gastric cancer(GC)and its relationship with GC cells'proliferation,migration,invasion,and epi-thelial-mesenchymal transition(EMT).Methods:Cancerous and paracancerous tissue sections from 42 GC patients were collected,and the expression of PNMA was detected by immunohistochemical staining.The GC cell lines SGC-7901,HGC27,N87,NU1,BGC-823,and the human normal gastric mucosal epithelial cell line GES-1 were cultured,and the expression level PNMA5 was detected by Western blot.Stable overexpres-sion of PNMA5 in N87 cells(PNMA5-OE-N87)and negative control cells(PNMA5-OENC-N87),as well as stable knockdown of PNMA5 in HGC27 cells(shPN5-HGC27)and its negative control cells(shPNMA5-NC-HGC27)were constructed by lentivirus transfection.Subsequently the proliferation activity of each group of cells was detected by CCK-8 assay and colony formation assay,the migration activity of each group of cells was detected by assay,the invasive activity of each group of cells was detected by Transwell assay,and the ex-pression levels of EMT-related proteins Vimentin,E-cherin,and N-cadherin were detected by Western blot.The nude mouse GC xenograft models were constructed to observe the effect of PNMA overexpression or knock-down on tumor growth and EMT level in vivo.Results:Compared with adjacent tissues,PNMA5 was upregu-lated in cancer tissues(P<0.05).Compared with ES-1 cells,PNMA5 was upregulated in all GC cell lines(all P<0.05).Compared with PNMA5-OENC-N87 cells,PNMA5-OE-N87 cells had significantly in-creased proliferation,migration,and invasion activities,with elevated Vimentin and N-cherin expression lev-els and reduced E-cadherin expression level(all P<0.05);compared with shPNMA5-NC-H27 cells,shPN-MA5-HGC27 cells had significantly reduced proliferation,migration,and invasion activities,with reduced Vimentin and N-cherin expression levels and elevated E-cadherin expression level(all P<0.05).In vivo ex-periments showed that overexpression of PN5 promoted GC tumor growth and EMT level,while knockdown of PNMA5 inhibited tumor growth and EMT level(all P<0.05).Conclusion:PNMA5 is upregulated in GC and promotes GC cells proliferation,migration,invasion,and EMT,acting as an oncogene in GC.关键词
胃癌/副肿瘤抗原Ma家族成员5/增殖/迁移/侵袭/上皮-间质转化Key words
Gastric cancer/Paraneoplastic antigen Ma family member 5/Proliferation/Migra-tion/Invasion/Epithelial-mesenchymal transition引用本文复制引用
黄丽华,左毅,韦珍平,黄雨菲,李彩兰,耿焱..PNMA5在胃癌组织中的表达及其与胃癌细胞恶性生物学行为的关系研究[J].河北医学,2025,31(7):1082-1087,6.基金项目
广西壮族自治区卫生厅计划课题,(编号:Z-A20231095) （编号:Z-A20231095）
