河北医学2025，Vol.31Issue(7)：1087-1092,6.DOI:10.3969/j.issn.1006-6233.2025.07.06
基于miR-146a/TRAF6轴探讨LncRNA NORAD对非霍奇金淋巴瘤细胞焦亡增殖和侵袭的影响
Effect of LncRNA NORAD on Pyroptosis Proliferation and Invasion of Non-Hodgkin Lymphoma Cells Based on miR-146a/TRAF6 Axis
摘要
Abstract
Objective:To investigate the effect of lncRNA NORAD on pyroptosis,proliferation and inva-sion of non-Hodgkin's lymphoma cells based on miR-146a/TRAF6 axis.Methods:NHL cell lines Jurkat,Raji and normal B cells were cultured.The expression levels of lncRNA NORAD,miR-146a and TRAF6 were detected by quantitative real-time polymerase chain reaction(qRT-PCR).Raji cell line was selected for lncRNA NORAD knockdown treatment(NORAD-siNC group,NORAD-si group).Western blot was used to detect the expression levels of NOD-like receptor protein 3(NLRP3),Cleaved caspase1,Gasdermins-DN terminal domain(N-GSDMD)and TRAF6.qRT-PCR was used to detect the expression level of miR-146a.The levels of interleukin-1β(IL-1β)and IL-18 in cell culture supernatant were detected by kits.EDU as-say was used to detect cell proliferation activity,and Transwell assay was used to detect cell invasion activity.Then,the correlation between lncRNA NORAD and miR-146a,and between miR-146a and TRAF6 was ver-ified by dual-luciferase activity detection.Results:Compared with normal B cells,the expression of lncRNA NORAD and TRAF6 was increased in Jurkat and Raji cells,while the expression of miR-146a was decreased.Compared with cells in the NORAD-siNC group,cells in the NORAD-si group had increased expression lev-els of miR-146a,NLRP3,N-GSDMD and Cleaved caspase1,decreased expression level of TRAF6,weak-ened cell proliferation and invasion activities,and increased levels of IL-1β and IL-18 in cell culture super-natant.The results of dual-luciferase assay showed that there were target binding relationships between ln-cRNA NORAD and miR-146a,and between miR-146a and TRAF6.Conclusion:lncRNA NORAD is up-regulated in NHL cells.Knocking down the expression of lncRNA NORAD can induce pyroptosis of NHL cells and inhibit cell proliferation and invasion through the miR-146a/TRAF6 axis.关键词
长链非编码RNA NORAD/非霍奇金淋巴瘤/细胞焦亡/微小RNA 146a/肿瘤坏死因子受体相关蛋白6Key words
Long non-coding RNA NORAD/Non-Hodgkin lymphoma/Pyroptosis/MicroRNA 146a/Tumor necrosis factor receptor-associated protein 6引用本文复制引用
李杨,刁玉巧,王江娅,朱秀丽,江莲..基于miR-146a/TRAF6轴探讨LncRNA NORAD对非霍奇金淋巴瘤细胞焦亡增殖和侵袭的影响[J].河北医学,2025,31(7):1087-1092,6.基金项目
2023年度河北省医学科学研究课题计划,(编号:20230915) （编号:20230915）
