河北医学2025，Vol.31Issue(7)：1092-1097,6.DOI:10.3969/j.issn.1006-6233.2025.07.07
温阳通痞消积方通过调控线粒体凋亡途径对肝癌小鼠细胞凋亡的影响
The Effect of Wenyang Tongpi Xiaoji Prescription on the Apoptosis of Liver Cancer Mouse Cells by Regulating the Mitochondrial Apoptosis Pathway
摘要
Abstract
Objective:To investigate whether the Wenyang Tongpi Xiaoji prescription(WYTP)can promote apoptosis of liver cancer mouse cells by regulating the mitochondrial apoptosis pathway.Methods:H22 liver cancer cells were cultured,and a nude mouse model of H22 liver cancer transplantation was estab-lished.The nude mice were assigned to a control group,a positive drug group,a low-dose WYTP(WYTP-L)group,a medium-dose WYTP(WYTP-M)group,and a high-dose WYTP(WYTP-H)group.Tumor growth was observed,and tumor inhibition rate was calculated.HE staining was used to observe the morpholo-gy of tumor tissues.Mitochondrial membrane potential(MMP)was analyzed by mitochondrial red fluorescent probe.TUNEL staining was performed to detect cell apoptosis in tumor tissues.Western blot was used to de-tect the protein expression levels of Cleaved-Caspase-9,Caspase-9,Caspase-3,Cleaved-Caspase-3,Cyt C,Bax,Bcl-2,and PCNA.Results:The survival status of nude mice in each group was basically good.Compared with the control group,the tumor cells of nude mice in the WYTP-L group,WYTP-M group,WYTP-H group,and positive drug group showed loose arrangement,varying degrees of nuclear condensation and vacuolization,and cell necrosis,the tumor mass,tumor volume,mitochondrial membrane potential,the protein expression levels of Bcl-2 and PCNA decreased,the tumor inhibition rate,number of apoptotic tumor cells,and expression levels of Cleaved-Caspase-9,Caspase-9,Caspase-3,Cleaved-Caspase-3,Cyt C,and Bax proteins in tumor tissue cells increased(P<0.05).There was no statistically prominent difference in the above indicators between the positive drug group and the WYTP-H group(P>0.05).Conclusion:WYTP can promote apoptosis of liver cancer mouse cells and inhibit cell proliferation by regulating the mito-chondrial apoptosis pathway.关键词
肝癌细胞/温阳通痞消积方/线粒体凋亡途径/凋亡/线粒体膜电位Key words
Hepatocellular carcinoma cells/Wenyang Tongpi Xiaoji prescription/Mitochondrial apoptosis pathway/Apoptosis/Mitochondrial membrane potential引用本文复制引用
杨春,向彩琼,覃鑫,邹丹,张军,郑新平,徐大志..温阳通痞消积方通过调控线粒体凋亡途径对肝癌小鼠细胞凋亡的影响[J].河北医学,2025,31(7):1092-1097,6.基金项目
湖北省中医药科研立项项目,(编号:ZY2023F095) （编号:ZY2023F095）
荆州市科技局项目,(编号:2024HD168) （编号:2024HD168）
