河北医学2025，Vol.31Issue(7)：1102-1108,7.DOI:10.3969/j.issn.1006-6233.2025.07.09
LncRNA MALAT1靶向miR-106a-5p/Smad7对IL-1β诱导的软骨细胞损伤的影响
Impact of LncRNA MALAT1 on IL-1β-Induced Chondrocyte Injury by Targeting miR-106a-5p/Smad7
摘要
Abstract
Objective:To investigate the impact of long non-coding RNA(LncRNA)metastasis associ-ated lung adenocarcinoma transcript 1(MALAT1)on interleukin-1β(IL-1β)-induced chondrocyte injury,and to determine whether it is related to microRNA-106a-5p(miR-106a-5p)/mother against decapentaple-gic homolog 7(Smad7).Methods:The human chondrocyte line C20/A4 was used as the research subject,and a chondrocyte injury model was induced by IL-1β.The cells were assigned into IL-1β group,IL-1β+sh-NC group,IL-1β+sh-MALAT1 group,IL-1β+sh-MALAT1+inhibitor NC group,and IL-1β+sh-MALAT1+miR-106a-5p inhibitor group.The control group was C20/A4 without IL-1β induction.QRT-PCR was performed to detect the mRNA expression of LncRNA MALAT1,miR-106a-5p,and Smad7.MTT and flow cytometry were performed to detect cell proliferation and apoptosis.ELISA was performed to detect IL-6,IL-8,and IL-10.Dual luciferase reporter assay was used to validate the targeting relationship between miR-106a-5p with LncRNA MALAT1 and Smad7.In addition,Western blot was used to detect apoptosis related proteins.Results:Compared with the control group,the IL-1β group showed increased LncRNA MALAT1,Smad7 mRNA expression,cell proliferation inhibition rate,apoptosis rate,IL-6,IL-8 levels,and Bax pro-tein expression,and decreased miR-106a-5p expression,IL-10 level,and Bcl-2 protein expression(P<0.05).Compared with the IL-1β group and the IL-1β+sh-NC group,the IL-1β+sh-MALAT1 group had re-duction in LncRNA MALAT1,Smad7 mRNA expression,cell proliferation inhibition rate,apoptosis rate,IL-6,IL-8 levels,and Bax protein expression,and elevation in miR-106a-5p expression,IL-10 level,and Bcl-2 protein expression(P<0.05).Compared with the IL-1β+sh-MALAT1 group and the IL-1β+sh-MALAT1+inhibitor NC group,the IL-1β+sh-MALAT1+miR-106a-5p inhibitor group showed no significant difference in LncRNA MALAT1 expression(P>0.05),reduced miR-106a-5p expression,IL-10 level,and Bcl-2 protein expression,and elevated Smad7 mRNA expression,cell proliferation inhibition rate,apoptosis rate,IL-6,IL-8 levels,and Bax protein expression(P<0.05).MALAT1 targeted the regulation of miR-106a-5p/Smad7 axis.Conclusion:Silencing MALAT1 can upregulate miR-106a-5p and inhibit Smad7,thereby promoting cell proliferation,inhibiting cell apoptosis and inflammatory response,and alleviating IL-1β-induced chondrocyte injury.关键词
LncRNA MALAT1/miR-106a-5p/Smad7/IL-1β/软骨细胞Key words
LncRNA MALAT1/miR-106a-5p/Smad7/IL-1β/Chondrocyte引用本文复制引用
白波,马佳良,刘永权,张玉舰,陈国栋..LncRNA MALAT1靶向miR-106a-5p/Smad7对IL-1β诱导的软骨细胞损伤的影响[J].河北医学,2025,31(7):1102-1108,7.基金项目
河北省秦皇岛市科学技术研究与发展计划项目,(编号:202301A251) （编号:202301A251）
