河北医学2025，Vol.31Issue(7)：1121-1127,7.DOI:10.3969/j.issn.1006-6233.2025.07.012
miR-93-5p通过靶向ROCK2对狼疮性肾炎肾小球系膜细胞损伤的影响
Effects of miR-93-5p on Renal Mesangial Cell Damage in Lupus Nephritis by Targeting ROCK2
摘要
Abstract
Objective:To investigate the effects of microRNA-93-5p(miR-93-5p)on renal mesang-ial cell(RMC)damage in lupus nephritis(LN)mice.Methods:QRT-PCR was used to detect the expres-sion of miR-93-5p and ROCK2 mRNA in the serum of 33 LN patients(LN group)treated in our hospital from July 2023 to May 2024 and 33 pairs of healthy individuals(control group)undergoing physical examina-tion.RMCs(identified by morphology)were isolated and purified from LN mice and assigned into Control group,NC-mimics group(transfected with NC-mimics),miR-93-5p-mimics group(transfected with miR-93-5p-mimics),miR-93-5p-mimics+OE-NC group(transfected with miR-93-5p-mimics+OE-N),and miR-93-5p-mimics+OE-ROCK2 group(transfected with miR-93-5p-mimics+OE-ROCK2).The expres-sion of miR-93-5p and ROCK2 mRNA was detected in the cell lysates of each group.MTT and flow cytome-try were used to detect the effects of overexpression of miR-93-5p on RMC proliferation and apoptosis,re-spectively.ELISA kit was used to detect the expression of TNF-α,IL-6,and IL-1β in in the supernatant of RMC cells.In addition,Western blot was used to detect the expression of PCNA,Bcl-2,Cleaved Caspase-3,and ROCK proteins in the lysate of RMC cells.Results:Compared with control group,LN group showed a decrease in miR-93-5p(t=15.977,P<0.001)and an increase in ROCK2(t=11.292,P<0.001).Com-pared with the Control group and the NC-mimics group,the values of A570,the expressions of miR-93-5p,PCNA and Bcl-2 in the miR-93-5p-mimics group were increased.The expressions of apoptosis rate,ROCK2 mRNA and protein,TNF-α,IL-6,IL-1β,and Cleaved Caspase-3 decreased(P<0.05).Com-pared with miR-93-5p-mimics group and miR-93-5p-mimics+OE-NC group,the miR-93-5p-mimics+OE-ROCK2 group showed a decrease in A570 value,miR-93-5p,PCNA,and Bcl-2,and an increase in apoptosis rate,ROCK2 mRNA and protein,TNF-α,IL-6,IL-1β,and Cleaved Caspase-3 expression(P<0.05).Conclusion:miR-93-5p inhibits RMC damage in LN mice by targeting ROCK2.关键词
狼疮性肾炎/微小RNA-93-5p/Rho激酶2/肾小球系膜细胞/细胞损伤Key words
Lupus nephritis/MicroRNA-93-5p/Rho kinase 2/Renal mesangial cells/Cell damage引用本文复制引用
王静,刘佳,何立芳,宋玉竹,薛艳云..miR-93-5p通过靶向ROCK2对狼疮性肾炎肾小球系膜细胞损伤的影响[J].河北医学,2025,31(7):1121-1127,7.基金项目
河北省医学科学研究重点课题项目,(编号:20231838) （编号:20231838）
