河北医学2025，Vol.31Issue(7)：1134-1139,6.DOI:10.3969/j.issn.1006-6233.2025.07.014
电针联合健脾理气法通过TLR4/NF-κB信号通路对脓毒症大鼠肠道菌群紊乱的改善作用
Effect of Electroacupuncture Combined with Spleen-Invigorating and Qi-Regulating Method on Intestinal Flora Disorder in Sepsis Rats via TLR4/NF-κB Signaling Pathway
摘要
Abstract
Objective:To explore the improvement effect of electroacupuncture combined with spleen-invigorating and qi-regulating formula on intestinal flora disorder in sepsis rats and its possible molecular mechanism based on the TLR4/NF-κB signaling pathway.Methods:SD rats were divided into sham operation group,model group,electroacupuncture group,spleen and qi formula group,and electroacupuncture+spleen and qi formula group,and the sepsis rat model was constructed by cecal ligation and perforation.HE staining was used to detect pathological damage of colon tissues.ELISA was applied to detect serum levels of TNF-α,IL-6,and IL-1β.16S rRNA sequencing was performed to analyze intestinal flora.Western blot was used to detect the expression of TLR4,p-p65 NF-κB/p65 NF-κB,p-IκB and other proteins related to the TLR4/NF-κB signaling pathway in colon tissues.Results:Compared with the sham operation group,the model group showed obvious pathological damage in colon tissues,significantly increased serum levels of TNF-α,IL-6,and IL-1β(P<0.05),and decreased alpha diversity indices(observed_species,Chao1,Shannon,Simpson)of intestinal flora(P<0.05).At the phylum level,the relative abundance of Proteobacteria in-creased,while that of Firmicutes and Bacteroidetes decreased.At the genus level,the relative abundances of Alloprevotella and Bifidobacterium(related to short-chain fatty acid production and anti-inflammation)de-creased,while those of Escherichia-Shigella and Klebsiella(related to lipopolysaccharide production)in-creased.The expression of TLR4,p-p65 NF-κB/p65 NF-κB,and p-IκB proteins in colon tissues signifi-cantly increased(P<0.05).Compared with the model group,the electroacupuncture group and spleen-invig-orating and qi-regulating formula group showed alleviated pathological damage in colon tissues,significantly decreased serum levels of TNF-α,IL-6,and IL-1β(P<0.05),increased alpha diversity indices of intesti-nal flora(P<0.05),increased relative abundances of Firmicutes,Bacteroidetes,Alloprevotella,and Bifidobacterium,decreased relative abundances of Proteobacteria,Escherichia_Shigella,and Klebsiella,and significantly decreased expression of TLR4,p-p65 NF-κB/p65 NF-κB,and p-IκB proteins in colon tissues(P<0.05).The effect of electroacupuncture combined with spleen-invigorating and qi-regulating formula was significantly better than that of single electroacupuncture or formula(P<0.05).Conclusion:Electroacupunc-ture combined with spleen-invigorating and qi-regulating formula may improve intestinal flora disorder and in-hibit inflammation by inhibiting the TLR4/NF-κB signaling pathway,thereby reducing intestinal damage in sepsis rats,and the combination of the two therapeutic methods has a synergistic effect.关键词
脓毒症/电针/健脾理气方/TLR4/NF-κB/肠道菌群Key words
Sepsis/Electroacupuncture/Spleen and Qi formula/TLR4/NF-κB/Gut micro-biota引用本文复制引用
臧红敏,崔颖,董妍..电针联合健脾理气法通过TLR4/NF-κB信号通路对脓毒症大鼠肠道菌群紊乱的改善作用[J].河北医学,2025,31(7):1134-1139,6.基金项目
河北省中医药管理局科研计划项目,(编号:2022065) （编号:2022065）
