河南农业科学2025，Vol.54Issue(8)：69-81,13.DOI:10.15933/j.cnki.1004-3268.2025.08.007
硅藻土和生物炭对稻田甲烷排放及相关微生物的影响
Impact of Diatomite and Biochar Application on Methane Emissions and Related Microorganisms in Paddy Fields
摘要
Abstract
To clarify the effects of diatomite,biochar,and their combined application on methane(CH4)emission,methanogen(mcrA)and methanotroph(pmoA)communities in paddy fields,a pot experiment was set up with four treatments:no amendment(CK),full-dose biochar(B),full-dose diatomite(S),and half-dose biochar plus half-dose diatomite(BS).During the entire rice growth period,CH4 emissions were monitored,and soil dissolved organic carbon(DOC)content,ammonium nitrogen(NH4+-N)content,redox potential(Eh),as well as mcrA and pmoA gene abundances and related microbial community structures were determined and compared.The results showed that compared with CK,the total cumulative CH4 emissions in B,S,and BS treatments decreased by 23.47%,29.95%,and 32.51%throughout the growth period.Compared with CK,S and BS treatments significantly increased soil NH4+-N content.The abundances of mcrA and pmoA genes and the mcrA/pmoA value were the highest in the tillering stage across all four treatments.Compared with CK,the average mcrA/pmoA values in B,S,and BS treatments decreased by 40.82%,49.78%,and 55.31%throughout the growth period.The community diversity of methanogens increased,while that of methanotrophs decreased in B,S,and BS treatments.These treatments reduced the relative abundances of Methanosarcinaceae and Methylococcus,but increased that of Methanobacteriaceae;S and BS treatments further increased the relative abundance of Methylocystis.Correlation analysis results indicated that CH4 emission flux was significantly positively correlated with the mcrA/pmoA value at the significance levels of 0.001,0.01,0.05,and 0.05 in the tillering,jointing,booting,and maturity stages,respectively.In conclusion,biochar,diatomite,and their combined application all have the potential to reduce CH4 emissions in paddy fields,with the combined application of biochar and diatomite exhibiting the best CH4 reduction effect.关键词
稻田/生物炭/硅藻土/甲烷/产甲烷菌/甲烷氧化菌Key words
Paddy field/Biochar/Diatomite/Methane/Methanogen/Methanotroph分类
农业科技引用本文复制引用
田黎霞,李双龙,刘章勇,刘冬,蒋梦蝶,聂江文,金涛..硅藻土和生物炭对稻田甲烷排放及相关微生物的影响[J].河南农业科学,2025,54(8):69-81,13.基金项目
国家自然科学基金项目(42377287) （42377287）
国家重点研发计划项目(2022YFD2301000) （2022YFD2301000）
