寄生虫与医学昆虫学报2025，Vol.32Issue(2)：65-72,8.DOI:10.3969/j.issn.1005-0507.2025.02.001
PD-1缺失对旋毛虫感染小鼠体内免疫反应的影响
EFFECT OF PD-1 DEFICIENCY ON IMMUNE RESPONSE IN MICE INFECTED WITH TRICHINELLA SPIRALIS
摘要
Abstract
Objective To investigate the effect of programmed death-1(PD-1)on cell infiltration in muscle tissue and immune response types in mice infected with Trichinella spiralis.Methods C57BL/6J wild-type(WT)and PD-1 deficient(PD-1-/-)mice were infected with T.spiralis(400 muscle larvae per mouse),and samples were collected on day 35 after infection.The proportions of infiltrating inflammatory cells and fibroblasts around encapsulated larvae were assessed by immunohistochemistry.The expression levels of interferon-γ(IFN-γ),interleukin(IL)-4,IL-5,IL-13,and eotaxin in muscle tissue were measured using enzyme-linked immunosorbent assay.Peripheral blood and spleen were collected at different time points after infection.The percentages of CD4+IFN-γ+Th1 and CD4+IL-4+Th2 within CD4+T cells population in peripheral blood and spleen of mice were analyzed using flow cytometry.Results The proportions of eosinophils and fibroblasts among total infiltrating cells around the encapsulated larvae in the muscle of PD-1-/-mice were significantly lower than those in WT mice after T.spiralis infection(P＜0.01).The infected PD-1-/-mice exhibited higher proportions of macrophages,T cells and B cells in total infiltrating cells than the infected WT mice(P＜0.01).The levels of IL-4,IL-5,IL-13,and eotaxin in the muscle tissue of infected PD-1-/-mice were significantly lower than those in infected WT mice(P＜0.05).However,IFN-γ levels were not significantly different between the infected WT and PD-1-/-mice.The proportions of Th2 cells in CD4+T cells from peripheral blood and spleen of infected PD-1-/-mice were significantly lower than those in infected WT mice,whereas the proportion of Th1 cells showed no difference among the infected groups.Conclusions PD-1 deletion results in decreased expression of key chemokines of eosinophils and key cytokines of fibroblast formation,and a corresponding decrease in inflammatory cells in muscle in T.spiralis-infected mice.This effect may be associated with a diminished Th2 immune response caused by PD-1 deletion.关键词
旋毛虫/程序性死亡分子-1/2型辅助性T细胞/嗜酸性粒细胞Key words
Trichinella spiralis/Programmed death-1/T helper 2 cell/Eosinophils分类
医药卫生引用本文复制引用
赵思蒙,黄欣扬,刘祎琪,张瑶,于艳,黄京京,诸欣平,程喻力..PD-1缺失对旋毛虫感染小鼠体内免疫反应的影响[J].寄生虫与医学昆虫学报,2025,32(2):65-72,8.基金项目
北京市自然科学基金项目(7202008) （7202008）
国家自然科学基金项目(No.81572016) （No.81572016）
