寄生虫与医学昆虫学报2025，Vol.32Issue(2)：93-98,6.DOI:10.3969/j.issn.1005-0507.2025.02.005
抑制田鼠巴贝斯虫增殖的蜱自噬分子的鉴定
IDENTIFICATION OF THE TICK AUTOPHAGY MOLECULE INHIBITING THE PROLIFERATION OF BABESIA MICROTI
摘要
Abstract
Objective Ticks serve as vectors for transmitting Babesia microti.However,the specific mechanism remains unclear.This study aimed to investigate the effect of tick autophagy molecules on the proliferation of Babesia microti.Methods An experimental model of infected and uninfected mice was used to collect tick materials for proteomic analysis to identify differentially expressed autophagy-related molecules in Haemaphysalis longicornis.The cloning of the HlATG8 gene,protein expression,and production of polyclonal antibodies were completed.The HlATG8 gene was then knocked down using RNAi interference technology.Results The tick autophagy molecule,HlATG8,was identified and found to be significantly upregulated in ticks infected with Babesia microti.The load of Babesia microti in ticks increased significantly following the knockdown of the HlATG8 gene.Conclusions The tick autophagy molecule in Hae.longicornis,HlATG8,inhibits the proliferation of Babesia.关键词
田鼠巴贝斯虫/长角血蜱/自噬/ATG8Key words
Babesia microti/Haemaphysalis longicornis/Autophagy/ATG8分类
医药卫生引用本文复制引用
龚凤君,曹杰,周勇志,王亚楠,张厚双,周金林..抑制田鼠巴贝斯虫增殖的蜱自噬分子的鉴定[J].寄生虫与医学昆虫学报,2025,32(2):93-98,6.基金项目
国家重点研发计划项目(2022YFD1800200) （2022YFD1800200）
