实用临床医药杂志2025，Vol.29Issue(13)：27-32,38,7.DOI:10.7619/jcmp.20252079
CXC趋化因子配体5通过激活Wnt/β-连环蛋白通路促进胃癌免疫逃逸及移植瘤生长的机制研究
Mechanism of CXC chemokine ligand 5 in promoting immune escape and transplanted tumor growth in gastric cancer via activating Wnt/β-catenin pathway
摘要
Abstract
Objective To investigate the effect of CXC chemokine ligand 5(CXCL5)on the growth of transplanted tumors of gastric cancer(GC)cells in mice via Wnt/β-catenin signaling path-way.Methods A total of 70 GC patients(GC group)and 70 healthy individuals(healthy group)were selected as study subjects.The serum CXCL5 levels in each group were detected,and the posi-tive rate of CXCL5 expression in GC tissues was determined using immunohistochemical staining.The relationship between CXCL5 expression and clinicopathological features was analyzed.Forty mice were divided into overexpressed CXCL5 group and control group,with 20 mice in each group.The mouse gastric cancer cell line MFC was used to establish a mouse transplanted tumor model by stable overexpression of CXCL5 and transfection with an empty vector.The growth of transplanted tumors and the survival conditions of mice in each group were recorded.The proportions of CD4+T lympho-cytes,CD8+T lymphocytes,and regulatory T cells(Treg cells)in the transplanted tumor tissues of mice were detected using flow cytometry.The expressions of CXCL5 and Wnt/β-catenin signaling pathway-related proteins in the transplanted tumor tissues of mice were detected using Western blot.The mRNA expressions of cyclin D1 and proto-oncogene c-Myc(c-Myc)in the transplanted tumor tissues of mice in each group were detected using reverse transcription-quantitative real time poly-merase chain reaction(RT-qPCR).Results The serum CXCL5 level in the GC group was higher than that in the healthy group,and the difference was statistically significant(P＜0.05).The posi-tive rate of CXCL5 expression in GC tissues was 67.14％(47/70),which was higher than 35.71％(25/70)in adjacent tissues,and the difference was statistically significant(P＜0.05).There were significant differences in tumor differentiation degree,TNM stage,and lymph node metastasis be-tween GC patients with positive and negative CXCL5 expressions(P＜0.05).The survival rate of mice in the overexpressed CXCL5 group was lower than that in the control group,and the difference was statistically significant(P=0.048).As time elapsed,the tumor volume of mice in the overex-pressed CXCL5 group was larger than that in the control group,and the difference was statistically significant(P＜0.05).The protein expressions of CXCL5,Wnt1,Wnt2,and β-catenin in the tumor tissues of mice in the overexpressed CXCL5 group were higher than those in the control group,and the differences were statistically significant(P＜0.05).The proportions of CD4+T cells and CD8+T cells in the tumor tissues of mice in the overexpressed CXCL5 group were lower than those in the control group,while the proportion of Treg cells was higher than that in the control group,and the differences were statistically significant(P＜0.05).The mRNA expressions of Cyclin D1 and c-Myc in the tumor tissues of mice in the overexpressed CXCL5 group were higher than those in the control group,and the differences were statistically significant(P＜0.05).Conclusion The ex-pression levels of CXCL5 in cancer tissues and serum of GC patients are upregulated.Abnormally high expression of CXCL5 may activate the Wnt/β-catenin signaling pathway,affect tumor immune activity,and promote the progression of GC.关键词
胃癌/CXC趋化因子配体5/Wnt/β-连环蛋白/肿瘤免疫/Treg细胞/靶向治疗/肿瘤微环境/细胞周期蛋白D1Key words
gastric cancer/CXC chemokine ligand 5/Wnt/β-catenin/tumor immunity/Treg cells/targeted therapy/tumor microenvironment/cyclin D1分类
医药卫生引用本文复制引用
赵元洋,徐萍,王建华..CXC趋化因子配体5通过激活Wnt/β-连环蛋白通路促进胃癌免疫逃逸及移植瘤生长的机制研究[J].实用临床医药杂志,2025,29(13):27-32,38,7.基金项目
国家自然科学基金项目(31700737) （31700737）
