军事医学2025，Vol.49Issue(7)：486-493,8.DOI:10.7644/j.issn.1674-9960.2025.07.002
长链非编码RNA神经素-2反义RNA 1调控肝癌细胞增殖和侵袭及对有氧糖酵解的影响
Long non-coding RNA NRSN2-AS1 regulates proliferation and invasion of liver cancer cells as well as aerobic glycolysis
摘要
Abstract
Objective To investigate the biological function and potential mechanisms of long non-coding RNA(lncRNA)neurensin 2-antisense RNA 1(NRSN2-AS1)in liver cancer cells.Methods The Encyclopedia of RNA Interactomes(ENCORI)database was used to analyze the expression levels of NRSN2-AS1 in liver cancer tissues and normal tissues as well as its association with the prognosis of patients.Stable lncRNA NRSN2-AS1 cell lines,overexpressed or knockdown,were constructed.The effects of NRSN2-AS1 on tumor cell proliferation were explored using CCK-8 and colony formation assays.Transwell and wound healing assays were employed to examine the role of NRSN2-AS1 in tumor cell migration and invasion.The impact of NRSN2-AS1 on tumor cell aerobic glycolysis was assessed by measuring hexokinase activity,glucose uptake,ATP and etracellular lactate levels.Quantitative real-time PCR(qPCR)and Western blotting were used to evaluate the effects of NRSN2-AS1 on the mRNA and protein expression levels of hexokinase 2(HK2)in tumor cells.Results Analysis from the ENCORI database revealed that NRSN2-AS1 was upregulated in liver cancer tissues compared to normal tissues,and that high expressions of NRSN2-AS1 were closely associated with poor prognosis of patients.In vitro functional assays demonstrated that overexpression of NRSN2-AS1 promoted proliferation,migration,and invasion of liver cancer cells,and enhanced glycolysis levels while knockdown of NRSN2-AS1 inhibited these processes and suppressed glycolysis.Furthermore,overexpression of NRSN2-AS1 increased the mRNA and protein levels of HK2 while knockdown of NRSN2-AS1 decreased HK2 expression in liver cancer cells.Conclusion NRSN2-AS1 is highly expressed in liver cancer tissues,and it may promote liver cancer progression by enhancing HK2 expression and aerobic glycolysis.关键词
神经素-2反义RNA 1/己糖激酶Ⅱ/肝细胞癌/增殖/转移/有氧糖酵解Key words
neurensin 2-antisense RNA 1/hexokinase 2/hepatocellular carcinoma/proliferation/metastasis/aerobic glycolysis分类
医药卫生引用本文复制引用
吴淑蒙,杨圆君,王振,吴淑慧,成敏英,李玲,叶棋浓..长链非编码RNA神经素-2反义RNA 1调控肝癌细胞增殖和侵袭及对有氧糖酵解的影响[J].军事医学,2025,49(7):486-493,8.基金项目
国家自然科学基金项目(81930078、82330083) （81930078、82330083）
国家重点研发计划项目(2022YFA1104003) （2022YFA1104003）
