菌物学报2025，Vol.44Issue(8)：129-143,15.DOI:10.13346/j.mycosystema.250032
香叶基香叶基焦磷酸合成酶在银耳芽孢中的表达及代谢调节
Expression and metabolic regulation of geranylgeranyl diphosphate synthase in the yeast-like conidia of Tremella fuciformis
摘要
Abstract
Geranylgeranyl pyrophosphate synthetase(GGPPS)is a key enzyme in the synthesis of secondary metabolites of plant diterpenes.By expressing GGPPS of Taxus chinensis in yeast-like conidia(YLC)of Tremella fuciformis,the regulation of exogenous GGPPS genes on terpene metabolism pathway,nutrition and active metabolites of YLC of T.fuciformis was investigated for the purpose of providing scientific theoretical basis for the expression of terpene compounds by using YLC of T.fuciformis as biological reactors.The exogenous gene GGPPS was heterologously expressed using lithium acetate conversion method.The production of terpenoid geranyl geranyl pyrophosphate(GGPP)from engineered YLC of T.fuciformis was analyzed for the first time,and the regulation of terpenoid metabolism pathway,nutrition and active metabolites by exogenous genes GGPPS in engineered strains were analyzed.The diterpene compound precursor GGPP was detected in the engineered strain YLC of T.fuciformis.Compared with the control strain,the content of isopentenyl pyrophosphate(IPP),dimethylallyl pyrophosphate(DMPP),farnesyl pyrophosphate(FPP),squalene and ergosterol in the terpenoid metabolic pathway of the engineered strain YLC of T.fuciformis were significantly decreased,while the total diterpene content was increased.The content of metabolites such as nutrient components(total protein,amino acid composition,trace elements,carbohydrates)and active substances(total polysaccharide,reducing sugar,soluble sugar)were changed.The exogenous gene GGPPS can utilize substrates in the terpenoid metabolic pathway of YLC of T.fuciformis to generate the diterpene compound precursor GGPP,thereby regulating the metabolic flux of the mevalonate pathway in the terpenoid metabolic pathway,as well as changes in various metabolites of nutritional components and active substances.关键词
银耳芽孢/香叶基香叶基焦磷酸合成酶/香叶基香叶基焦磷酸/萜类代谢/营养成分Key words
YLC of Tremella fuciformis/geranylgeranyl pyrophosphate synthase/geranylgeranyl pyrophosphate/terpenoid metabolism/nutrients引用本文复制引用
辛燕花,郭丽琼,林俊芳..香叶基香叶基焦磷酸合成酶在银耳芽孢中的表达及代谢调节[J].菌物学报,2025,44(8):129-143,15.基金项目
国家自然科学基金(31071837) （31071837）
广东省粤港联合创新领域项目(2014B050505018) （2014B050505018）
山西省科技厅项目(2021030 2124375) （2021030 2124375）
忻州市科技局重点研发项目(20220212)This work was supported by the National Natural Science Foundation of China(31071837),the Guangdong Hong Kong Joint Innovation Field Project(2014B050505018),the Fundamental Research Program of Shanxi Province(20210302124375),and the Key Research&Development Project of Xinzhou Science and Technology Bureau(20220212). （20220212）
