菌物学报2025，Vol.44Issue(8)：157-166,10.DOI:10.13346/j.mycosystema.250021
不同碳氮源及前体物质对金顶侧耳菌丝体生物量和麦角硫因含量影响
Effects of various carbon and nitrogen sources as well as precursor substance on mycelial biomass and ergothioneine content of Pleurotus citrinopileatus
摘要
Abstract
Ergothioneine is a natural antioxidant with strong functions of anti-oxidation,free radical scavenging,and metal ion chelation.Pleurotus citrinopileatus is rich in ergothioneine.In this study,the biomass and ergothioneine content of P.citrinopileatus strains were used as main indicators and the optimal carbon and nitrogen sources of fermentation medium were screened out for obtaining high-yield mycelium biomass and ergothioneine.At the same time,the excellent strains were screened out,and the effects of adding different precursor substances on the biomass and ergothioneine content of P.citrinopileatus strains were investigated.The results showed that using lactose as the carbon source and casein peptone as the nitrogen source,the mycelium biomass and ergothioneine content of the four strains,P.citrinopileatus No.1,No.2,No.3 and No.83520,reached the highest value.P.citrinopileatus No.1 has the highest mycelium biomass and ergothioneine content,reaching 1.52 g/100 mL and 106.81 μg/mL respectively.The optimal precursor substance was methionine.After addition of methionine,the mycelial biomass of P.citrinopileatus No.1 was 1.98 g/100 mL,and the ergothioneine content was 228.908 μg/mL,being double of those before addition.关键词
金顶侧耳/菌丝体生物量/麦角硫因/碳氮源/前体物质Key words
Pleurotus citrinopileatus/mycelium biomass/ergothioneine/carbon and nitrogen source/precursor substance引用本文复制引用
王琳,王语聪,姜水地,谢智鑫,刘丹怡,全润,高志威,刘容旭,韩建春..不同碳氮源及前体物质对金顶侧耳菌丝体生物量和麦角硫因含量影响[J].菌物学报,2025,44(8):157-166,10.基金项目
黑龙江省自然科学基金(LH2022C084) （LH2022C084）
省属科研院所科研业务费(CZKYF2024-1-C012) （CZKYF2024-1-C012）
大学生SIPT创新创业训练项目(202410224187)This work was supported by the Natural Science Foundation Project of Heilongjiang Province(LH2022C084),the Research Fund of Provincial Research Institutes in Heilongjiang Province(CZKYF2024-1-C012),and the SIPT Innovation and Entrepreneurship Training Program for College Students(202410224187). （202410224187）
