菌物学报2025，Vol.44Issue(8)：167-181,15.DOI:10.13346/j.mycosystema.250003
具抑菌活性的橙黄黑孢霉卷线孢菌素高产发酵工艺优化
Optimization of fermentation process of Nigrospora aurantiaca for high production of bostrycin with antibiotic activities
摘要
Abstract
To increase the yield of bostrycin from Nigrospora aurantiaca GDYX-311,an endophytic fungus of Ginkgo biloba,a study based on combining single factor experiment and response surface method is carried out for optimization of fermentation medium formulation and culture conditions of the fungus.The antibacterial and antifungal activities of bostrycin in vitro are also carried out.The results showed that fermentation time,temperature and inoculum size had significant effects on bostrycin yield.The optimal media were glucose 50 g/L,beef extract powder 10 g/L,and MgSO4·7H2O 0.3 g/L.Under the optimal fermentation time of 129.12 h,temperature of 27.38℃,inoculum size of 0.81%,and natural pH of 5.74,the yield of bostrycin was(414.89±11.10)µg/mL,2.06 times of the yield under unoptimized condition[(201.55±18.90)µg/mL].The antibacterial and antifungal experiment found that bostrycin has significant antibiotic effects on Shigella castellani,Candida albicans,Salmonella typhimurium and Streptococcus agalactiae.This study provides reference for large-scale liquid fermentation of bostrycin and its antibacterial and antifungal application.关键词
橙黄黑孢霉/卷线孢菌素/液体发酵/抑菌活性Key words
Nigrospora aurantiaca/bostrycin/liquid fermentation/antibiotic activities引用本文复制引用
李慧,马召迪,雷帮星,陈其荣,何张江,刘正慧,康冀川..具抑菌活性的橙黄黑孢霉卷线孢菌素高产发酵工艺优化[J].菌物学报,2025,44(8):167-181,15.基金项目
国家自然科学基金(32170019,32460007)This work was supported by the National Natural Science Foundation of China(32170019,32460007). （32170019,32460007）
