菌物研究2025，Vol.23Issue(4)：295-303,9.DOI:10.13341/j.jfr.2023.1665
元蘑苦味相关氧化鲨烯环化酶基因克隆及表达模式分析
Cloning and Expression Pattern Analysis of Bitterness-Related Oxidosqualene Cyclase Gene in Sarcomyxa edulis
摘要
Abstract
In order to reveal the role of oxidosqualene cyclase (OSC) genes in the bitter flavor formation of Sarcomyxa edulis,we obtained the sequence of SeOSC gene through PCR cloning,on the basis of former transcriptome analysis with strains having different levels of bitterness,and performed the structural analysis of protein as well as the real-time quantitative PCR to investigate the expression pattern of SeOSC in different growth stages of S.edulis.The SeOSC gene has a length of 2 232 bp,encoding 743 amino acids which has been considered as a kind of hydrophilic and unstable protein.Multiple sequence alignment and phylogenetic tree analysis revealed that the SeOSC protein shares more than 80％ of sequence homology with that of Ganoderma lingzhi and Hypsizygus marmoreus.Furthermore,the SeOSC protein is found to be species-specific and showed the closest genetic relationship with that of Hypsizygus marmoreus.The expression of SeOSC gene was detected at all different stages of S.edulis through growth to development,but displayed clearly variable levels among the strains.Notably,the bitter T115 strain showed a much higher level of SeOSC gene expression compared to that of the non-bitter T184 strain,in which the gene expression activity in the bitter T115 strain was approximately ten times greater than that of the non-bitter T184 strain during the middle growth stage of fruiting-body.This fact indicates that the significantly different expression patterns of the SeOSC gene identified between strains with different levels of bitterness might be correlated with the biological synthesis activity of bitter substances(P＜0.01).Our above results should provide a fundamental basis for further analysis of the functional role of SeOSC gene in synthesis mechanism of bitter substances in S.edulis.关键词
食药用菌/氧化鲨烯环化酶/基因克隆/苦味风味Key words
edible-medicinal fungi/oxidosqualene cyclase/gene clone/bitter flavor分类
农业科技引用本文复制引用
覃再丽,谢江涛,彭科琴,梅世惠,李长田,田风华,李玉..元蘑苦味相关氧化鲨烯环化酶基因克隆及表达模式分析[J].菌物研究,2025,23(4):295-303,9.基金项目
国家自然科学基金项目(NSFC No.32000013) （NSFC No.32000013）
