菌物研究2025，Vol.23Issue(4)：313-321,9.DOI:10.13341/j.jfr.2023.1628
3株野生赤芝的生物学特性及灵芝酸类成分含量的测定
Biological Characterization and Detection of Ganoderic Acids in Three Wild Ganoderma sichuanense Strains
摘要
Abstract
Ganoderma sichuanense is a kind of macrofungi with high medicinal and economic values.For the extensive exploration and utilization of its natural resources,an analysis of internal transcribed spacer sequences(ITS)was conducted in this study on three wild Ganoderma strains collected respectively from Fujian and Zhejiang provinces in China,all of which were identified as belonging to the species of G.sichuanense.The above three wild strains of G.sichuanense(S-Ⅱ,S-Ⅲ,S-Ⅳ)together with one major domestic strain(S-Ⅰ)were used as research materials for biological characterization,cultivation trial,and detection of main ganoderic acids.Best growth conditions seemed to differ among individual strains of G.sichuanense with an optimal temperature of 30 ℃,an optimal carbon source of starch,an optimal nitrogen source of yeast extract,and an optimal pH value of 5.Cultivation experiments using artificial substitutes demonstrated that all of the three wild G.sichuanense strains were able to generate fruiting bodies and conduct sporulation in normal.Analysis of chemical substances within the fruiting bodies of tested strains by using ultra-performance liquid chromatography combined with quadruple-time-of-flight mass spectrometry(UPLC-QTOF-MS)led to the successful detection of 18 ganoderic acids that have already been reported to carry extensive pharmacological effects.It was very interesting to know from this study that the wild strain S-Ⅳrevealed its content of both 13 individual ganoderic acids or total ganoderic acids higher than that of cultivated strain.The above successful cultivation of three wild strains and discovery of one wild strain with a high content of ganoderic acids should provide available references for the future development of wild germplasm resources and breeding of new varieties of G.sichuanense.关键词
赤芝/种质资源/生物学特性/灵芝酸Key words
Ganoderma sichuanense/germplasm resource/biological characteristics/ganoderic acids分类
农业科技引用本文复制引用
吕艳聪,李玉,张国亮,熊辉,徐靖,李振皓,孙慧颖..3株野生赤芝的生物学特性及灵芝酸类成分含量的测定[J].菌物研究,2025,23(4):313-321,9.基金项目
国家自然科学基金项目(32270028),吉林省科技发展计划项目(20220202109NC),企事业单位委托科技项目(GF20220570) （32270028）
