解放军医学院学报2025，Vol.46Issue(3)：247-255,9.DOI:10.12435/j.issn.2095-5227.25010301
LRFN4通过谷氨酰胺代谢途径影响胃癌细胞增殖
LRFN4 regulates gastric cancer cell proliferation through glutamine metabolism
许尹梅 1付彦堪 1怀思远 1游路宽 1李建雄1
作者信息
- 1. 解放军总医院第五医学中心放射治疗科,北京 100071
- 折叠
摘要
Abstract
Background LRFN4 is a pivotal molecule in the genesis and progression of cancer.Nevertheless,its function in gastric cancer(GC)and its correlation with glutamine catabolism remain elusive.Objective To explore the clinical significance and prognostic predictive value of LRFN4 in gastric cancer,and verify its impact on glutamine metabolism in gastric cancer cell lines through in-vitro cellular experiments.Methods Bioinformatics approaches were utilized to analyze the influence of LRFN4 on the clinical prognosis and other aspects of gastric cancer patients,by means of databases such as TCGA,GEPIA,and Kaplan-Meier Plotter,along with R language software.Gastric cancer cell lines were employed to detect glutamine metabolic activity via glutamine uptake and glutaminase activity assays,CCK-8 assay for cell proliferation,and flow cytometry for cell apoptosis,aiming to verify the biological functions of LRFN4 on gastric cancer cells.Results Differential analysis revealed that LRFN4 expression was markedly upregulated in gastric cancer tissues(P<0.05).Prognostic analysis indicated that both the overall survival and disease-free survival of gastric cancer patients in the high-expression group were lower than those in the low-expression group(P<0.05),suggesting that LRFN4 holded guiding significance for the prognosis of gastric cancer patients.LRFN4 might affect the occurrence and development of gastric cancer through signaling pathways like CELL CYCLE,promote cell proliferation(P<0.05),and inhibit cell apoptosis(P<0.05)by influencing glutamine metabolism.Conclusion LRFN4 may be associated with the prognosis of gastric cancer patients by modulating glutamine metabolism,functioning as an oncogenic driver in gastric cancer cells,promoting tumor cell proliferation,and inhibiting cell apoptosis.关键词
LRFN4/胃癌/预后/谷氨酰胺代谢/肿瘤代谢Key words
LRFN4/gastric cancer/prognosis/glutamine metabolism/cancer metabolism分类
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许尹梅,付彦堪,怀思远,游路宽,李建雄..LRFN4通过谷氨酰胺代谢途径影响胃癌细胞增殖[J].解放军医学院学报,2025,46(3):247-255,9.
