临床输血与检验2025，Vol.27Issue(4)：492-498,7.DOI:10.3969/j.issn.1671-2587.2025.04.009
东莞地区CD36抗原缺失型血小板献血者资料库的建立与分析
Establishment and Analysis of the Database of CD36 Antigen-deficient Platelet Donors in Dongguan
摘要
Abstract
Objective To establish a database of CD36 antigen-deficient platelet donors in Dongguan and analyze the characteristics.Methods Samples from 565 blood donors were tested for platelet CD36 antigen expression using flow cytometry.The gene sequences of 13 CD36-deficient samples and 187 samples from blood donors included in the database were analyzed using the third-generation gene sequencing(TGS).Results Flow cytometry detected CD36 deficiency in 13 cases,yielding a deficiency rate of 2.30%(13/565).Of these,2 cases were type Ⅰ deficient(15.38%)and 11 cases were typeⅡ deficient(84.62%).There was no significant difference in the rate of CD36 antigen deletion based on gender(P＞0.05),ethnicity(Han vs.minority groups,P＞0.05),or ABO blood group distribution(P＞0.05).TGS revealed mutations in 7 of the 13 CD36 deficient samples,including 5 exons and 2 intron regions.Both type Ⅰ cases showed no detectable mutations,while all type Ⅱ cases exhibited heterozygous mutations.Additionally,27 CD36 gene mutations were identified in 187 CD36 normal donors.The mutation rate in CD36 deficient donors(53.85%,7/13)was significantly higher than that of CD36 normal donors(14.44%,27/187)(P＜0.05).Conclusion The frequency of platelet CD36 deficiency in Dongguan is higher than that reported in Chongqing but consists with findings from other Chinese regions.Establishing a serologically validated CD36-deficient platelet donor database in the area is recommended to enhance clinical transfusion safety.关键词
血小板/CD36/基因/献血者/血小板资料库Key words
Platelets/CD36/Gene/Blood donor/Platelet donor database分类
医药卫生引用本文复制引用
何子毅,胡应明,贝孟辉,刘静,揭小梅,陈少彬,钟炽辉,梁华钦..东莞地区CD36抗原缺失型血小板献血者资料库的建立与分析[J].临床输血与检验,2025,27(4):492-498,7.基金项目
本课题受东莞市社会科技发展重点项目(No.20231800936012)、广州市医学重点学科项目(No.2025-2027)资助 （No.20231800936012）
