上海预防医学2025，Vol.37Issue(6)：490-495,6.DOI:10.19428/j.cnki.sjpm.2025.24707
2019-2022年上海市宝山区腹泻患者弯曲菌耐药性及分子分型研究
Antibiotic resistance and molecular typing of Campylobacter spp.from diarrheal patients in Baoshan District of Shanghai,2019-2022
摘要
Abstract
[Objective]To analyze the drug resistance and the molecular typing characteristics through pulsed field gel electrophoresis(PFGE)of Campylobacter spp.isolated from patients with infectious diarrhea in Baoshan District of Shanghai,and to provide a basis for Campylobacter spp.prevention and control and clinical medication.[Methods]Campylobacter spp.was isolated,cultured and identified from stool samples of diarrheal patients collected from medical institutions at two monitoring sites in Baoshan District from 2019 to 2022.Antimicrobial susceptibility testing for 12 antibiotics was conducted on the isolated Campylobacter jejuni(C.jejuni)and Campylobacter.Coli(C.coli),and molecular typing was performed using PFGE.[Results]A total of 179 strains of Campylobacter spp.were isolated from 1 786 samples of diarrheal patients,with a positive rate of 10.02%.The highest resistance rate of C.jejuni was to ciprofloxacin(98.63%),followed by tetracycline(97.26%)and nalidixic acid(89.73%).C.coli was completely resistant to ciprofloxacin and nalidixic acid(100.00%),followed by tetracycline(90.91%).The multidrug resistance rates of C.jejuni and C.coli were 89.73%and 100.00%,respectively.142 strains of C.jejuni produced 122 PFGE bands,while 33 strains of C.coli produced 33 PFGE bands,and the distribution of the bands was relatively dispersed.[Conclusion]From 2019 to 2022,the detection rate of Campylobacter in diarrheal patients was relatively high in Baoshan District of Shanghai,the multidrug resistance rate of Campylobacter isolates from diarrheal patients was relatively serious,in addition,the drug resistance pattern was complex,and the PFGE band pattern displayed a polymorphic distribution.关键词
弯曲菌/腹泻/检出率/脉冲场凝胶电泳/耐药性Key words
Campylobacter/diarrhea/detection rate/pulsed field gel electrophoresis/antibiotic resistance分类
医药卫生引用本文复制引用
牛娜,崔世勇,沈隽卿,张旭,金敏,唐小德..2019-2022年上海市宝山区腹泻患者弯曲菌耐药性及分子分型研究[J].上海预防医学,2025,37(6):490-495,6.基金项目
宝山区传染病流行病学重点学科项目(BSZK-2023-A20) （BSZK-2023-A20）
