生物技术通报2025，Vol.41Issue(8)：65-73,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0339
RUBY辅助的水稻高效CRISPR基因编辑
Efficient CRISPR Gene Editing in Rice Assisted by RUBY
摘要
Abstract
[Objective]To develop a RUBY-assisted CRISPR/Cas9 gene-editing vector(GCR)enabling visual screening of transgenic plants and gene-editing events.[Method]The GCR vector was constructed by coupling RUBY with the Cas9/gRNA expression cassette.Using OsAGO2,OsAGO3,and OsAGO7 in rice(Oryza sativa)as target genes,two multi-gene editing vectors(GCR-237-1 and GCR-237-2)were designed.These vectors were introduced into rice(Zhonghua 11,ZH11)via Agrobacterium-mediated genetic transformation to generate gene-edited plants.[Result]After the transformation of GCR-237-1 and GCR-237-2 into rice callus,RUBY markers effectively indicated transgenic positive events,and the target genes of stable transformed red plants were efficiently edited.[Conclusion]The GCR vector enables effective gene editing in rice,and transgenic or gene-edited plants can be rapidly identified through direct visual observation.关键词
遗传转化/基因编辑/CRISPR/RUBY/水稻Key words
genetic transformation/gene editing/CRISPR/RUBY/rice(Oryza sativa)引用本文复制引用
邓美壁,严浪,詹志田,朱敏,和玉兵..RUBY辅助的水稻高效CRISPR基因编辑[J].生物技术通报,2025,41(8):65-73,9.基金项目
国家自然科学基金项目(32200335),三亚中国农业科学院国家南繁研究院南繁专项(YBXM2446,YBXM2505) （32200335）
