生物技术通报2025，Vol.41Issue(8)：146-154,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0104
大麦条纹病菌GH17基因家族的鉴定及表达分析
Identification and Expression Analysis of GH17 Gene Family of Pyrenophora graminea
摘要
Abstract
[Objective]Glycoside hydrolase 17(GH17)plays a significant role in various physiological and pathogenic processes in plants,animals and fungi.The identification and preliminary analysis of the PgGH17 family members in the Pyrenophora graminea provide a scientific basis for further in-depth research on the function of GH17 genes,and concurrently lay a solid theoretical foundation for understanding the function of pathogenicity genes in the P.graminea and exploring the host-pathogen interaction mechanism.[Method]Using P.graminea as the research subject,we identified and analyzed gene families through bioinformatics approaches.Subsequently,real-time quantitative PCR(RT-qPCR)was employed to monitor the temporal expression patterns of these genes during the infection process.[Result]The PgGH17 gene family comprises six members,with amino acid sequence lengths ranging from 305 to 648 aa.All proteins are hydrophilic,exhibiting isoelectric points(pI)of 5.06‒8.66 and aliphatic indices of 65.43‒82.00.Signal peptide lengths vary from 0 to 22 aa,with most members localized to the extracellular matrix;only one gene harbors a transmembrane domain.Intron numbers range from 1 to 3 per gene.Phylogenetic analysis revealed that these family members cluster into seven distinct subclades.Quantitative reverse transcription PCR(RT-qPCR)results demonstrated that,with the exception of PgGH17-3,the expressions of other PgGH17 genes during infection generally present first an initial upregulation followed by subsequent downregulation.[Conclusion]Six GH17 family members are identified in P.graminea,and with the exception of GH17-3,all show upregulated expressions during infection,suggesting their functional involvement in the pathogenicity of this fungal pathogen.关键词
大麦条纹病菌/糖苷水解酶17(GH17)/基因家族/基因表达分析Key words
Pyrenophora graminea/glycoside hydrolase 17(GH17)/gene family/gene expression analysis引用本文复制引用
任睿斌,孟亚雄,司二静,万广有,汪军成,姚立蓉,张宏,马小乐,李葆春,王化俊..大麦条纹病菌GH17基因家族的鉴定及表达分析[J].生物技术通报,2025,41(8):146-154,9.基金项目
国家大麦青稞产业技术体系项目(CARS-05-03B-03),国家自然科学基金项目(31960426,32160496),甘肃省自然科学基金重点项目(24JRRA637) （CARS-05-03B-03）
