生物技术通报2025，Vol.41Issue(8)：165-174,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0208
基于SCoT、SRAP和SSR分子标记的220份辣椒种质资源遗传多样性分析
Genetic Diversity of 220 Pepper Germplasm Resources Using SCoT,SRAP,and SSR Molecular Markers
摘要
Abstract
[Objective]To comprehensively analyze the genetic diversity and phylogenetic relationships of pepper germplasm resources preserved at the Ningxia Academy of Agriculture and Forestry Sciences,providing a theoretical foundation for the conservation,introduction,and application of pepper germplasm resources.[Method]Genetic diversity analysis was conducted on 220 pepper germplasm resources from 15 regions across China using three molecular marker techniques:SSR,SRAP,and SCoT.[Result]Six SCoT markers amplified 1 to 4 bands,14 SRAP markers amplified 2 to 4 bands,and 3 SSR markers amplified 2 to 5 bands,with all markers showing 100%polymorphic band percentages.Further analysis revealed that SSR markers had the highest average polymorphic information content(PIC)of 0.77,significantly higher than SCoT(0.55)and SRAP(0.55),indicating that SSR markers were more advantageous for detecting genetic diversity,with HpmsE088 being the most effective SSR marker.Cluster analysis,principal coordinate analysis,and population genetic structure analysis divided the germplasm from the 15 regions into two major groups(Group 1 and Group 2).Group 1 included germplasm from Sichuan,Shenyang,Xinjiang,Shandong,Ningxia,and Hunan in China,while Group 2 included germplasm from China(Yunnan,Jiangsu,Henan,Gansu,Beijing,and Anhui),Netherlands,Japan,and the United States.Additionally,83.18%of the germplasm demonstrated high homozygosity.A correlation was observed between fruit shape characteristics and cluster grouping,with a significantly higher proportion of linear fruit pepper germplasm in Group 1 compared to Group 2,while all short horn-shaped fruit pepper germplasm were classified into Group 2.[Conclusion]The pepper germplasm resources preserved at the Ningxia Academy of Agriculture and Forestry Sciences show high genetic diversity and can be primarily divided into two groups based on genetic information.The findings provide a theoretical basis for future introduction strategies and parental selection.关键词
辣椒/遗传多样性/SCoT标记/SRAP标记/SSR标记Key words
pepper/genetic diversity/SCoT marker/SRAP marker/SSR marker引用本文复制引用
裴红霞,汪露瑶,李生梅,高晶霞..基于SCoT、SRAP和SSR分子标记的220份辣椒种质资源遗传多样性分析[J].生物技术通报,2025,41(8):165-174,10.基金项目
宁夏农林科学院科技创新先导-科技攻关项目(NKYG-25-18),宁夏自然科学基金项目(2023AAC03424) （NKYG-25-18）
