生物技术通报2025，Vol.41Issue(8)：255-266,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0013
薄荷UV-B受体基因McUVR8的克隆与表达分析
Cloning and Expression Analysis of the UV-B Receptor Gene McUVR8 in Mentha canadensis L
摘要
Abstract
[Objective]The ultraviolet-B(UV-B)receptor UVR8 plays a key role in regulating plant growth and development in response to adversity stress.The aim is to explore the protein properties and expression characteristics of McUVR8,a UV-B receptor gene in mint(Mentha canadensis L.),and to predict its interacting proteins,thus to provide theoretical references for the subsequent probing of the function of McUVR8.[Method]The McUVR8 gene was obtained by RT-PCR,and its physicochemical properties,protein structure and subcellular localization,and interacting proteins were analyzed and predicted using a variety of bioinformatics tools.Tissue and leaf sequence expression patterns of McUVR8 and its expression changes under different hormones and stresses were analyzed using RT-qPCR.[Result]McUVR8 is 1 353 bp in length,encoding 450 amino acids,distributed in both nucleus and cytoplasm,with conserved helix-looped RCC1 core domain and C17 and C27 domains,and there is high homology with SsUVR8 of Salvia splendens,SdBUR8 of Salvia divinorum and ShUVR8 of Salvia hispanica,which are also in the Labiaceae family.McUVR8 was expressed in different tissues and leaf sequences of M.canadensis,with some differences,with the lowest expression in the roots,the highest expression in the flowers,and the highest expression in the first leaf in different leaf sequences.In addition,the McUVR8 gene responded to MeJA,ABA,and drought,NaCl,and two heavy metal and aluminum stress treatments in both leaves and roots.Finally,McUVR8 was screened and verified to interact with McCOP1.[Conclusion]McUVR8 may have important roles in regulating mint growth and development,responding to hormonal or adversity signals,and it may be involved in regulating mint secondary metabolism,adversity response,and other processes by binding to COP1,WRKYs,and other interacting proteins.关键词
薄荷/UV-B受体McUVR8/蛋白特征/蛋白互作/表达模式/生信分析/基因克隆Key words
Mentha canadensis L/UV-B receptor McUVR8/protein characterization/protein interactions/expression pattern/raw letter analysis/gene cloning引用本文复制引用
康琴,汪霞,谌明洋,徐静天,陈诗兰,廖平杨,许文志,吴卫,徐东北..薄荷UV-B受体基因McUVR8的克隆与表达分析[J].生物技术通报,2025,41(8):255-266,12.基金项目
四川省科技教育联合基金面上项目(2024NSFSC2114),天府峨眉计划(1649),四川农业大学科研兴趣项目(20251838) （2024NSFSC2114）
