时珍国医国药2025，Vol.36Issue(15)：2801-2806,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-1501
基于Trib1探讨冠心康对动脉粥样硬化模型ApoE-/-小鼠棕色脂肪组织的调控作用
Exploring the regulatory effects of Guanxinkang(冠心康)on brown adipose tissue in ApoE-/-mice of atherosclerosis model based on Trib1
摘要
Abstract
Objective To investigate the potential mechanism by which Guanxinkang(冠心康,GXK)regulates adipose tissue browning in ApoE-/-mice with atherosclerosis(AS)model by observing its effect on Trib1 in adipose tissue.Methods ApoE-/-mice with high-fat diet(HFD)were used to establish an AS model,which were then randomly divided into the model group,low/high-dose GXK groups,and atorvastatin group,while C57BL/6J mice were selected as the control group.After intervention,contents of total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)in serum of each group were measured.Oil Red O stai-ning was used to observe the pathological changes of aortic sinus.HE staining was used to observe the pathological changes of adipose tis-sue.Western blot(WB)was performed to detect the relative protein expressions of peroxisome proliferators-activated receptor gamma coactivator 1 alpha(PGC1α),mitochondrial brown fat uncoupling protein-1(UCP1),Tribbles homolog 1(Trib1)in adipose tissue.Results Compared with the control group,the model group showed increased serum levels of TC,TG and LDL-C(P＜0.01),signifi-cantly enlarged aortic sinus plaques and adipocyte volume,disorganized cell arrangement,and decreased protein expressions of PGC1α,UCP1,and Trib1 in adipose tissue(P＜0.01).Compared with the model group,the high-dose GXK and atorvastatin groups exhibited reduced levels of serum TC,TG,and LDL-C(P＜0.05),decreased ratio of aortic sinus plaque to lumen area(P＜0.01),smaller adipocytes with orderly arrangement,and increased protein relative expressions of PGC1α,UCP1,and Trib1 in adipose tissue(P＜0.05).Conclusion GXK may ameliorate AS by promoting adipose tissue browning in ApoE-/-mice via mediating Trib1 in AS model.关键词
动脉粥样硬化/棕色脂肪组织/脂肪组织棕色化/冠心康/Trib1Key words
Atherosclerosis/Brown adipose tissue/Adipose tissue browning/Guanxinkang(冠心康)/Trib1分类
医药卫生引用本文复制引用
寇海芯,杨硕,常鑫迪,郭帅杰,张一凡,刘萍..基于Trib1探讨冠心康对动脉粥样硬化模型ApoE-/-小鼠棕色脂肪组织的调控作用[J].时珍国医国药,2025,36(15):2801-2806,6.基金项目
国家自然科学基金(82074200) （82074200）
上海市"科技创新行动计划"启明星计划(扬帆专项)(23YF1448100) （扬帆专项）
上海市浦东新区卫生健康委员会卫生计生科研项目(PW2022E-04) （PW2022E-04）
