时珍国医国药2025，Vol.36Issue(15)：2840-2846,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1507
基于IL-17信号通路研究益糖康对2型糖尿病糖脂紊乱的影响及作用机制
Study on the effect and mechanism of Yitangkang(益糖康)on glucose and lipid disorders in type 2 diabetes mellitus based on IL-17 signaling pathway
摘要
Abstract
Objective To investigate the effect and potential mechanism of the Chinese herbal medicine(CHM)compound Yitangkang(益糖康,YTK)on glucose and lipid metabolism disorders in type 2 diabetes mellitus(T2DM)based on the IL-17 signaling pathway.Methods Seventy-two SPF-grade male Wistar rats were randomly divided into the blank group,model group,YTK high/medium/low-dose groups and metformin hydrochloride group,with 12 in each group.After constructing a T2DM rat model,the patient was gavaged for 8 consecutive weeks.Fasting blood glucose(FBG)was measured using a Roche glucometer;fasting insulin(FINS)was detected by ELISA;triglyceride(TG),total serum cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipopro-tein cholesterol(LDL-C)were measured using an automatic biochemical analyzer;qRT-PCR and Western blot(WB)were used to detect the mRNA and protein expression levels of IL-17 signaling pathway-related genes in adipose tissue.Results The levels of FBG,TG,TC,and LDL-C in the high/medium-dose groups of YTK were significantly reduced,and the levels of HDL-C were sig-nificantly increased(P＜0.05).The mRNA and protein expression of IL-17,IL-17R,HSP90,TRAF6,MAPKs,AP-1,IL-6,TNF-α,and COX2 in the adipose tissue of rats were significantly reduced(P＜0.05);the mRNA expression levels of IL-17 and IL-17R in the high/medium-dose groups of YTK and the mRNA expression levels of HSP90,MAPKs,and COX2 in the high/medium-dose groups of YTK were significantly lower than those in the metformin hydrochloride group(P＜0.05).Conclusion YTK can alleviate T2DM glycolipid metabolic disorders and restore metabolic homeostasis,which may be associated with inhibiting the reaction between the key nodes of the IL-17 signaling pathway and cascade.Additionally,YTK shows a dosage-efficiency relationship in ameliorating T2DM glycolipid metabolic disorders.关键词
IL-17信号通路/2型糖尿病/中药复方益糖康/糖脂代谢紊乱Key words
IL-17 signaling pathway/Type 2 diabetes mellitus/CHM compound Yitangkang(益糖康)/Glucose and lipid me-tabolism disorders分类
医药卫生引用本文复制引用
张笑蕊,于嘉祥,程玥凤,石岩,张文顺..基于IL-17信号通路研究益糖康对2型糖尿病糖脂紊乱的影响及作用机制[J].时珍国医国药,2025,36(15):2840-2846,7.基金项目
国家重点基础研究发展计划(973计划)项目(2013CB532004) （973计划）
辽宁省科技厅联合基金项目(2023-MSLH-184) （2023-MSLH-184）
"中医脏象理论及应用"教育部重点实验室开放基金(zyzx2402) （zyzx2402）
辽宁省科技厅博士科研启动课题(2023-BS-141) （2023-BS-141）
辽宁省应用基础研究计划项目(2023JH2/101300094) （2023JH2/101300094）
