时珍国医国药2025，Vol.36Issue(15)：2847-2853,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1508
葛根舒筋方调控自噬治疗胶原诱导关节炎模型大鼠的作用机制研究
Study on the mechanism of Gegen Shujin Formula(葛根舒筋方)in treating collagen-induced arthritis rats via regulating autophagy
摘要
Abstract
Objective To investigate the pharmacological mechanism of Gegen(Radix Puerariae Lobatae)Shujin Formula(葛根舒筋方,GGSJF)in treating collagen-induced arthritis(CIA)rats by regulating autophagy.Methods Thirty male Wistar rats were random-ly divided into the normal group,model group,methotrexate group(7.6 mg·kg-1),and low/medium/high-dose GGSJF groups(4,8,16 g·kg-1),with 5 rats in each group.Except for the normal group,CIA models were established in all other groups by a secondary immunization method.The GGSJF groups and the methotrexate group were administered via intragastric gavage at their respective doses.Specifically,each GGSJF group received daily administration,while the methotrexate group was administered weekly,with continuous administration maintained for 28 consecutive days.After the experiment,body weight and paw thickness of rats were measured to evaluate the arthritis severity.Hematoxylin-eosin(HE)and safranin O-fast green staining were used to observe the pathological changes in knee joints.ELISA was used to detect the expressions of TNF-α,IL-6,IL-1 β,and IgG in serum.The expression levels of autoph-agy-related genes of Beclin-1,ATG5,ATG12,LC3B in ankle joints were measured by qPCR.Results Compared with the normal group,the model group showed decreased body weight,and red and swollen paws.Obvious synovial hyperplasia,inflammatory infiltra-tion,and cartilage damage could be observed through pathological staining of the knee joint.The expression levels of serum inflammatory factors TNF-α,IL-6,IL-1β,IgG,and autophagy-related genes Beclin-1,ATG5,ATG12,and LC3B in the ankle joint tissues were significantly increased compared with the normal group(P＜0.05).After drug intervention,all treatment groups exhibited symp-toms such as decreased body weight,and red and swollen paws,alleviated inflammatory infiltration in the knee joint and synovial hyper-plasia,and improved bone and cartilage structure.The expression levels of serum inflammatory factors TNF-α,IL-6,IL-1β,IgG,and autophagy-related gene Beclin-1,ATG5,ATG12,and LC3B in the ankle joint were significantly reduced compared to the model group,with the high-dose GGSJF and methotrexate groups showing the most pronounced effects(P＜0.05 or P＜0.01).Conclu-sion GGSJF may inhibit inflammatory responses and ameliorate CIA symptoms in rats by regulating autophagy.关键词
葛根舒筋方/胶原诱导关节炎/类风湿关节炎/自噬Key words
Gegen Shujin Formula(葛根舒筋方,GGSJF)/Collagen-induced arthritis(CIA)/Rheumatoid arthritis/Auto-phagy分类
医药卫生引用本文复制引用
李宜桓,庞彩霞,谭淑慧,阙嘉露,柳璇,周鹏,梁奇,刘翠玲..葛根舒筋方调控自噬治疗胶原诱导关节炎模型大鼠的作用机制研究[J].时珍国医国药,2025,36(15):2847-2853,7.基金项目
广东省基础与应用基础研究基金项目(2019A1515111192) （2019A1515111192）
深圳市宝安区科技创新局项目(2019JD316) （2019JD316）
深圳市"医疗卫生三名工程"项目(SZZYSM202206005) （SZZYSM202206005）
