时珍国医国药2025，Vol.36Issue(15)：2854-2860,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1509
基于线粒体凋亡途径探讨化浊解毒方对CRF大鼠的作用
To explore the mechanism of Huazhuo Jiedu Decoction(化浊解毒方)on CRF rats based on mitochondrial apoptosis pathway
摘要
Abstract
Objective To explore the mechanism of Huazhuo Jiedu Formula(化浊解毒方,HZJDF)/Turbidity-resolving and Toxins-removing Formula on 5/6 nephrectomy rats based on the mitochondrial apoptosis pathway.Methods The successfully modeled SD rats were divided into the model group,the HZJDF low-dose,medium-dose and high-dose groups,the losartan potassium group,with 9 rats in each group.The liver and kidney function of CRF rats was detected by biochemical method.The pathological changes of renal tis-sues were observed by HE staining and Masson staining.The ultrastructural changes of mitochondria in renal tubular epithelial cells were observed by transmission electron microscopy(TEM).The apoptosis rate of renal tissue cells was detected by Tunel staining.The protein and mRNA expression levels of Bel-2,Bax,Cyt C,Caspase-3 and Caspase-9 were detected by Western blot(WB)and Real-time PCR.Results Compared with the sham operation group,the values of Cr and BUN were significantly increased(P＜0.01).The me-sangial cells and stroma of the glomerulus were proliferating,the structure of the renal tubule interstitial area was disordered,a large number of blue collagen fibers were accumulated in the peribulbar and interstitial area,and the apoptosis rate of renal tissue cells was sig-nificantly increased(P＜0.01).Renal tubular epithelial cell mitochondria were swollen,ridge was fractured,vacuolar degeneration happened.The expression levels of Bel-2 protein and mRNA were significantly decreased(P＜0.05,P＜0.01),and the expression levels of Bax,Cyt C,Caspase-3,Caspase-9 protein and mRNA were significantly increased(P＜0.05,P＜0.01).After the inter-vention of HZJDF,the values of Cr and BUN were decreased significantly(P＜0.01).The above pathological damage was significantly relieved,the apoptosis rate of renal tissue cells decreased significantly(P＜0.01).The expression levels of Bcl-2 protein and mRNA were significantly increased(P＜0.05,P＜0.01),and the expression levels of Bax,Cyt C,Caspase-3,Caspase-9 protein and mR-NA were significantly decreased(P＜0.05,P＜0.01).Conclusion HZJDF can inhibit mitochondrial apoptosis by regulating Bcl-2/Bax pathway.关键词
5/6肾切除/慢性肾衰竭/化浊解毒方/肾功能/线粒体凋亡途径Key words
5/6 nephrectomy/Chronic renal failure/Huazhuo Jiedu Formula(化浊解毒方)/Renal function/Mitochondrial apoptosis pathway分类
医药卫生引用本文复制引用
高卉,郑琳琳,王婉卿,王雅诗,刘欣,魏晓娜..基于线粒体凋亡途径探讨化浊解毒方对CRF大鼠的作用[J].时珍国医国药,2025,36(15):2854-2860,7.基金项目
河北省自然科学基金项目(H2023423067) （H2023423067）
河北省中医药管理局科研计划项目(2025206) （2025206）
